公开(公告)号：US20190055513A1

公开(公告)日：2019-02-21

申请号：US16079070

申请日：2017-02-22

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Say Kong NG ,  Sow Zong Dawn LEONG ,  Gek Ling Janice TAN ,  Kah Hul Brian TEO

IPC: C12N5/00

Abstract: The invention relates to serum-free cell culture medium, wherein the medium either contains maltose as the sole carbohydrate source, or contains maltose and at least one other saccharide as carbohydrate sources. In a preferred embodiment, the additional saccharide is a monosaccharide, preferably glucose, and the medium comprises DMEM-F12. In a preferred embodiment the cells to be cultured may be CHO or HEK293 cells. Also disclosed are methods of growing and/or culturing a cell using the cell culture medium as described herein, methods of increasing protein yield using the cell culture medium as described herein, and kits thereof.

公开(公告)号：US20220154137A1

公开(公告)日：2022-05-19

申请号：US17534399

申请日：2021-11-23

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Say Kong NG ,  Sow Zong Dawn LEONG ,  Gek Ling Janice TAN ,  Kah Hui Brian TEO

IPC: C12N5/00

Abstract: The invention relates to serum-free cell culture medium, wherein the medium either contains maltose as the sole carbohydrate source, or contains maltose and at least one other saccharide as carbohydrate sources. In a preferred embodiment, the additional saccharide is a monosaccharide, preferably glucose, and the medium comprises DMEM-F12. In a preferred embodiment the cells to be cultured may be CHO or HEK293 cells. Also disclosed are methods of growing and/or culturing a cell using the cell culture medium as described herein, methods of increasing protein yield using the cell culture medium as described herein, and kits thereof.