公开(公告)号：US20190315800A1

公开(公告)日：2019-10-17

申请号：US16309889

申请日：2017-06-12

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley GAGNON ,  Yan Ling Phyllicia TOH ,  Wei ZHANG

IPC: C07K1/34

Abstract: This application describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid, thereby causing the formation of solids and removal of the solids using a suitable process. Further treatment by contacting the treated sample with a chemically reactive surface further enhances results achieved by the first purification step.