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    APPARATUS AND METHODS FOR FRACTIONATION OF BIOLOGICAL PRODUCTS
    1.
    发明申请
    APPARATUS AND METHODS FOR FRACTIONATION OF BIOLOGICAL PRODUCTS 审中-公开

    公开(公告)号：US20170173537A1

    公开(公告)日：2017-06-22

    申请号：US15124342

    申请日：2015-03-09

    申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    发明人： Peter Stanley GAGNON

    IPC分类号： B01D61/58 ,  C07K1/34 ,  C07K16/00 ,  B01D61/14

    摘要： Methods and apparatus for purification of biological molecules using combinations of tangential flow filtration subunits and adsorption sub-units in a single apparatus where the subunits may be operated independently or in series and where the apparatus and methods are suitable for use in a wide range of formats distinct from conditions required for chromatographic processes such as conducting process steps using adsorption subunits for purification of a biological molecule in a contaminated sample without prior buffer equilibration.

    EXOSOME RECOVERY METHODS WITH LOW MOLECULAR WEIGHT ORGANIC ZWITTERIONS
    5.
    发明申请
    EXOSOME RECOVERY METHODS WITH LOW MOLECULAR WEIGHT ORGANIC ZWITTERIONS 有权
    具有低分子量有机物的异常恢复方法

    公开(公告)号：US20160266097A1

    公开(公告)日：2016-09-15

    申请号：US15031660

    申请日：2014-10-15

    申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    发明人： Peter Stanley GAGNON

    IPC分类号： G01N33/50 ,  B01D15/36 ,  A61K35/12

    CPC分类号： G01N33/5076 ,  A61K35/12 ,  B01D15/364 ,  G01N33/5005

    摘要： A method of isolating exosomes includes conducting at least one purification step in the presence of an organic zwitterion having a molecular weight of less than about 350 Daltons, a buffering pK of a negatively charged portion of the organic zwitterion is at least one full pH unit below an operating pH at which the at least one purification step is conducted, and a buffering pK of the positively charged portion of the organic zwitterion is at least one full pH unit above the operating pH.

    摘要翻译： 分离外来体的方法包括在分子量小于约350道尔顿的有机两性离子存在下进行至少一个纯化步骤，有机两性离子的带负电荷部分的缓冲pK为至少一个完整的pH单位， 进行至少一个纯化步骤的操作pH，并且有机两性离子的带正电荷部分的缓冲pK是高于操作pH的至少一个完整的pH单位。

    CHROMATOGRAPHIC PURIFICATION OF VIRUS PREPARATIONS WITH NEGATIVELY CHARGED PARTICLES
    6.
    发明申请
    CHROMATOGRAPHIC PURIFICATION OF VIRUS PREPARATIONS WITH NEGATIVELY CHARGED PARTICLES 有权
    使用带负电荷的颗粒进行病毒制备的色谱纯化

    公开(公告)号：US20160002605A1

    公开(公告)日：2016-01-07

    申请号：US14768213

    申请日：2013-02-22

    申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    发明人： Peter Stanley GAGNON

    IPC分类号： C12N7/00 ,  B01D15/36 ,  B01D15/22 ,  B01D15/42 ,  B01D61/14

    CPC分类号： C12N7/00 ,  B01D15/22 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3847 ,  B01D15/424 ,  B01D61/145 ,  C12N2710/00051 ,  C12N2750/00051 ,  C12N2795/10151 ,  C12N2795/14151

    摘要： A method of purifying a sample that includes a desired virus includes the steps of (i) providing a packed chromatographic column having negatively charged porous particles, (ii) equilibrating the column to the conditions to which the desired virus in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume applied to the packed chromatographic column is less than or equal to the interparticle space of the negatively charged porous particles within the packed chromatographic column, (iv) eluting the desired virus from the packed chromatographic column, where the desired virus is in a purer state and in the conditions to which the packed chromatographic column was equilibrated.

    摘要翻译： 纯化包含所需病毒的样品的方法包括以下步骤：（i）提供具有带负电荷的多孔颗粒的填充色谱柱，（ii）使色谱柱平衡样品中所需病毒洗脱的条件， （iii）使样品与填充色谱柱接触，使得施加到填充色谱柱的样品体积小于或等于填充色谱柱内带负电荷的多孔颗粒的颗粒间间隔，（iv）洗脱所需病毒 从填充色谱柱中，所需病毒处于更纯的状态，并在填充色谱柱平衡的条件下进行。

    A METHOD OF ENHANCING THE PERFORMANCE OF CHROMATOGRAPHY METHODS FOR PURIFICATION OF PROTEINS
    7.
    发明申请
    A METHOD OF ENHANCING THE PERFORMANCE OF CHROMATOGRAPHY METHODS FOR PURIFICATION OF PROTEINS 审中-公开

    公开(公告)号：US20190315800A1

    公开(公告)日：2019-10-17

    申请号：US16309889

    申请日：2017-06-12

    申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    发明人： Peter Stanley GAGNON ,  Yan Ling Phyllicia TOH ,  Wei ZHANG

    IPC分类号： C07K1/34

    摘要： This application describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid, thereby causing the formation of solids and removal of the solids using a suitable process. Further treatment by contacting the treated sample with a chemically reactive surface further enhances results achieved by the first purification step.

    PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AT ELEVATED CONDUCTIVITY
    9.
    发明申请
    PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AT ELEVATED CONDUCTIVITY 审中-公开
    非线性有机聚合物在高电导率下的蛋白质纯化

    公开(公告)号：US20160009758A1

    公开(公告)日：2016-01-14

    申请号：US14769099

    申请日：2014-02-27

    申请人： Agency for Science, Technology and Research

    发明人： Peter Stanley GAGNON

    IPC分类号： C07K1/16

    CPC分类号： C07K1/16 ,  C07K1/30 ,  C07K16/065 ,  C07K16/36

    摘要： A method of purifying a desired protein from a preparation includes providing the preparation in a form having less than about 5% of the chromatin residing in the original production medium, contacting the preparation with a nonionic organic polymer in an amount sufficient to cause the desired protein to precipitate or adsorb on a nonionic hydrophilic surface, and adjusting a salt concentration before or during the contacting step, the adjusting step providing a sufficient salt concentration to produce a conductivity greater than physiological conductivity.

    摘要翻译： 从制备物纯化所需蛋白质的方法包括以低于存在于原始生产培养基中的染色质的约5％的形式提供制剂，使制剂与足以引起所需蛋白质的量的非离子有机聚合物接触 沉淀或吸附在非离子亲水表面上，并且在接触步骤之前或期间调节盐浓度，调节步骤提供足够的盐浓度以产生大于生理电导率的电导率。

    METHODS FOR REDUCING AGGREGATE CONTENT IN PROTEIN PREPARATIONS
    10.
    发明申请
    METHODS FOR REDUCING AGGREGATE CONTENT IN PROTEIN PREPARATIONS 有权
    降低蛋白质制剂中聚集体含量的方法

    公开(公告)号：US20150368354A1

    公开(公告)日：2015-12-24

    申请号：US14766129

    申请日：2014-02-05

    申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

    发明人： Peter Stanley GAGNON

    IPC分类号： C07K16/30 ,  B01D15/36 ,  B01D15/34 ,  C07K16/00 ,  C07K1/20

    CPC分类号： C07K1/14 ,  B01D15/327 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3828 ,  C07K1/165 ,  C07K1/20 ,  C07K16/00 ,  C07K16/3015

    摘要： A method of reducing aggregate and product-contaminant complex content in a protein preparation including a desired protein includes (i) contacting the protein preparation with at least one solid surface comprising at least one surface-bound ligand capable of binding a metal, the surface-bound ligand being capable of binding a metal is initially substantially devoid of a metal, the operating conditions are selected to substantially prevent the binding of the desired protein to the at least one solid surface and (ii) separating the protein preparation from the surface-bound ligand, such that when more than one surface-bound ligand is present, each surface-bound ligand is independently either of the same net charge or charge neutral.

    摘要翻译： 一种降低包含所需蛋白质的蛋白质制剂中的聚集体和产物 - 污染物复合物含量的方法包括（i）使蛋白质制剂与至少一个固体表面接触，所述至少一个固体表面包含至少一个能够结合金属的表面结合配体， 能够结合金属的结合配体最初基本上不含金属，选择操作条件以基本上防止所需蛋白质与至少一种固体表面的结合，和（ii）将蛋白质制剂与表面结合 配体，使得当存在多于一个表面结合的配体时，每个表面结合的配体独立地是相同的净电荷或电荷中性。