公开(公告)号：US10927145B2

公开(公告)日：2021-02-23

申请号：US16309889

申请日：2017-06-12

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Peter Stanley Gagnon ,  Yan Ling Phyllicia Toh ,  Wei Zhang

IPC: C07K1/34 ,  C07K1/36

Abstract: This application describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid, thereby causing the formation of solids and removal of the solids using a suitable process. Further treatment by contacting the treated sample with a chemically reactive surface further enhances results achieved by the first purification step.