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1.发明授权公开(公告)号：US10927145B2
公开(公告)日：2021-02-23
申请号：US16309889
申请日：2017-06-12
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Peter Stanley Gagnon , Yan Ling Phyllicia Toh , Wei Zhang
Abstract: This application describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid, thereby causing the formation of solids and removal of the solids using a suitable process. Further treatment by contacting the treated sample with a chemically reactive surface further enhances results achieved by the first purification step.
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公开(公告)号：US12246267B2
公开(公告)日：2025-03-11
申请号：US17440374
申请日：2020-03-20
Applicant: Agency for Science, Technology and Research
Inventor： Zi Ying Zheng , Wei Zhang
Abstract: There is provided a chromatography system comprising a first valve in fluid connection to a first chromatography column and/or a second valve; the second valve in fluid connection to a second chromatography column and the first valve; wherein the first valve and the second valve are operable to provide: a mode that selectively allows a fluid to flow from the first chromatography column to the second chromatography column; and one or more modes that selectively allows the fluid to bypass the first chromatography column and/or the second chromatography column. Also disclosed is a method of capturing and/or purifying a target from a sample thereof. In one embodiment, an acidic protein, alpha-1 anti-trypsin (A1AT), is purified by a tandem column configuration using anion exchange chromatography, whereby a first chromatography column is added in between the sample pump and an injection valve, or replaced the sample loop of AKTA system.
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公开(公告)号：US20220184526A1
公开(公告)日：2022-06-16
申请号：US17440374
申请日：2020-03-20
Applicant: Agency for Science, Technology and Research
Inventor： Zi Ying Zheng , Wei Zhang
Abstract: There is provided a chromatography system comprising a first valve in fluid connection to a first chromatography column and/or a second valve; the second valve in fluid connection to a second chromatography column and the first valve; wherein the first valve and the second valve are operable to provide: a mode that selectively allows a fluid to flow from the first chromatography column to the second chromatography column; and one or more modes that selectively allows the fluid to bypass the first chromatography column and/or the second chromatography column. Also disclosed is a method of capturing and/or purifying a target from a sample thereof. In one embodiment, an acidic protein, alpha-1 anti-trypsin (A1AT), is purified by a tandem column configuration using anion exchange chromatography, whereby a first chromatography column is added in between the sample pump and an injection valve, or replaced the sample loop of AKTA system.
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