公开(公告)号：US09234892B2

公开(公告)日：2016-01-12

申请号：US14273320

申请日：2014-05-08

Applicant: Agilent Technologies, Inc.

Inventor： May Tom-Moy ,  Nazumi Alice Yamada

IPC: G01N31/00 ,  G01N33/53 ,  G01N33/569

CPC classification number: G01N33/56966 ,  G01N33/582 ,  G01N2333/976

Abstract: A method for labelling a tissue section is provided. In certain embodiments, the method may comprise: (a) labeling a formalin-fixed paraffin embedded (FFPE) tissue section using a first set of labeling reagents that comprises a first primary antibody and a first labeled secondary antibody; (b) treating the labeled tissue with a protease, thereby digesting the first primary antibody and/or the first labeled secondary antibody and separating the label from the FFPE tissue section; (c) washing the tissue section to remove the separated label and the protease; and (c) labeling the FFPE tissue section using a second set of labeling reagents that comprises a second primary antibody and a second labeled secondary antibody. A kit for performing the method is also provided.

Abstract translation: 提供了一种用于标记组织切片的方法。 在某些实施方案中，该方法可以包括：（a）使用包含第一一抗和第一标记的二抗的第一组标记试剂标记福尔马林固定的石蜡包埋的（FFPE）组织切片; （b）用蛋白酶处理标记的组织，从而消化第一个一抗和/或第一个标记的二次抗体，并从FFPE组织切片分离标记物; （c）洗涤组织切片以除去分离的标签和蛋白酶; 和（c）使用包含第二个一级抗体和第二个标记的第二抗体的第二组标记试剂来标记FFPE组织切片。 还提供了用于执行该方法的套件。

公开(公告)号：US20150323532A1

公开(公告)日：2015-11-12

申请号：US14273320

申请日：2014-05-08

Applicant: Agilent Technologies, Inc.

Inventor： May Tom-Moy ,  Nazumi Alice Yamada

IPC: G01N33/569

CPC classification number: G01N33/56966 ,  G01N33/582 ,  G01N2333/976

Abstract: A method for labelling a tissue section is provided. In certain embodiments, the method may comprise: (a) labeling a formalin-fixed paraffin embedded (FFPE) tissue section using a first set of labeling reagents that comprises a first primary antibody and a first labeled secondary antibody; (b) treating the labeled tissue with a protease, thereby digesting the first primary antibody and/or the first labeled secondary antibody and separating the label from the FFPE tissue section; (c) washing the tissue section to remove the separated label and the protease; and (c) labeling the FFPE tissue section using a second set of labeling reagents that comprises a second primary antibody and a second labeled secondary antibody. A kit for performing the method is also provided.

Abstract translation: 提供了一种用于标记组织切片的方法。 在某些实施方案中，该方法可以包括：（a）使用包含第一一抗和第一标记的二抗的第一组标记试剂标记福尔马林固定的石蜡包埋的（FFPE）组织切片; （b）用蛋白酶处理标记的组织，从而消化第一个一抗和/或第一个标记的二次抗体，并从FFPE组织切片分离标记物; （c）洗涤组织切片以除去分离的标签和蛋白酶; 和（c）使用包含第二个一级抗体和第二个标记的第二抗体的第二组标记试剂来标记FFPE组织切片。 还提供了用于执行该方法的套件。

公开(公告)号：US20140212869A1

公开(公告)日：2014-07-31

申请号：US13750810

申请日：2013-01-25

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Nicholas M. Sampas ,  Robert A. Ach ,  Nazumi Alice Yamada ,  Kristin Bernick

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q2522/101 ,  C12Q2525/161 ,  C12Q2565/101 ,  C12Q2525/301 ,  C12Q2561/125

Abstract: Provided herein is a proximity assay that, in certain embodiments, involves: (a) hybridizing a first oligonucleotide and a second oligonucleotide with a target nucleic acid, wherein the first oligonucleotide comprises: i. a region that is complementary to a first sequence in the target nucleic acid and ii. a barcode sequence; and the second oligonucleotide comprises i. a region that is complementary to a second region in the target and ii. the complement of the barcode sequence; and (b) detecting hybridization between the barcode sequence and the complement of the barcode sequence, wherein hybridization between the barcode sequence and the complement of the barcode sequence indicates that the first and second target sequences are proximal to one another in the sample.

Abstract translation: 本文提供了在某些实施方案中的邻近测定法，其包括：（a）将第一寡核苷酸和第二寡核苷酸与靶核酸杂交，其中所述第一寡核苷酸包含： 与靶核酸中的第一序列互补的区域，和ii。 条形码序列; 并且第二寡核苷酸包括i。 与目标中的第二区域互补的区域，以及ii。 条形码序列的补码; 和（b）检测条形码序列与条形码序列的互补之间的杂交，其中条形码序列与条形码序列的互补之间的杂交表明第一和第二靶序列在样本中彼此靠近。