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    METHODS OF PROCESSING A SAMPLE FOR PEPTIDE MAPPING ANALYSIS
    1.
    发明公开
    METHODS OF PROCESSING A SAMPLE FOR PEPTIDE MAPPING ANALYSIS 审中-公开

    公开(公告)号：US20230349912A1

    公开(公告)日：2023-11-02

    申请号：US18026678

    申请日：2021-09-17

    申请人： AMGEN INC.

    发明人： Gang XIAO ,  Pavel BONDARENKO ,  Liuqing SHI ,  Thomas M. DILLON ,  Da REN ,  Margaret Speed RICCI ,  Weidong CUI ,  John Joseph HARRAHY ,  Jake PAWLOWSKI ,  Andrew DYKSTRA ,  Dylan RIGGS

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6821 ,  G01N33/6848 ,  G01N2333/976

    摘要： Provided herein are methods of processing a polypeptide or protein for analysis, e.g., peptide mapping analysis by mass spectrometry. In exemplary embodiments, the method comprises incubating a digested sample at a mildly acidic pH and/or in the presence of a chaotrope, wherein the digested sample is produced by digesting a polypeptide with a protease to produce a digested sample comprising at least two peptides. In exemplary embodiments, the method comprises digesting the polypeptide with a protease which cleaves C-terminal to tryptophan to produce a digested sample comprising at least two peptides. In exemplary embodiments, the method comprises digesting the polypeptide with trypsin at an enzyme:substrate (E:S) weight ratio of about 1:1 to about 1:15 to produce a digested sample comprising at least two peptides. In exemplary aspects, the digested sample comprises at least one or two peptides each comprising a tyrosine at the C-terminus.

    HYDROPHOBICITY SHIFTED ENZYME
    2.
    发明公开
    HYDROPHOBICITY SHIFTED ENZYME 审中-公开

    公开(公告)号：US20230258649A1

    公开(公告)日：2023-08-17

    申请号：US18167152

    申请日：2023-02-10

    申请人： Waters Technologies Corporation

    发明人： Matthew Lauber ,  Wenjing Li

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6821 ,  G01N2333/976

    摘要： The present invention relates to a method for analyzing a sample, the method comprising: (a) incubating a sample comprising an analyte with at least one enzyme to produce a digestion mixture comprising fragments of the analyte; (b) loading the digestion mixture onto a reversed-phase chromatography column; and (c) performing reversed-phase chromatography on the digestion mixture, wherein the at least one enzyme is hydrophobically modified to increase a retention time of the at least one enzyme such that the at least one enzyme elutes from the reversed-phase chromatography column later than the fragments of the analyte.

    METHODS AND COMPOSITIONS FOR THE TREATMENT OF SYMPTOMS OF PRION DISEASES
    4.
    发明申请
    METHODS AND COMPOSITIONS FOR THE TREATMENT OF SYMPTOMS OF PRION DISEASES 有权
    治疗重症疾病症状的方法和组合

    公开(公告)号：US20150273030A1

    公开(公告)日：2015-10-01

    申请号：US14713178

    申请日：2015-05-15

    申请人： Curemark, LLC

    发明人： Joan M. Fallon

    IPC分类号： A61K38/54 ,  C12Q1/37

    CPC分类号： A61K38/54 ,  A61K36/185 ,  A61K38/465 ,  A61K38/47 ,  A61K38/4826 ,  A61K38/4873 ,  C12Q1/37 ,  C12Y301/01 ,  C12Y302/01 ,  C12Y304/00 ,  C12Y304/21001 ,  C12Y304/21004 ,  C12Y304/22002 ,  G01N33/6896 ,  G01N2333/976 ,  G01N2800/2828 ,  G01N2800/52 ,  A61K2300/00

    摘要： A therapeutic composition for the treatment of the symptoms of prion diseases and the method for preparing the therapeutic agents is disclosed. The therapeutic composition is a stable pharmaceutical composition comprising one or more digestive and/or pancreatic enzymes. The therapeutic composition may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic composition may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using fecal chymotrypsin level as a biomarker for the presence of a prion disease, or the likelihood of an individual to develop a prion disease is disclosed.

    摘要翻译： 公开了治疗朊病毒疾病症状的治疗组合物和治疗剂的制备方法。 治疗组合物是包含一种或多种消化和/或胰酶的稳定的药物组合物。 治疗组合物可以通过各种封装技术制造。 治疗组合物的递送可以通过注射，通过粘附药物贴剂或其它方法口服制备。 此外，公开了使用粪便胰凝乳蛋白酶水平作为朊病毒疾病存在的生物标志物的方法或个体发展朊病毒病的可能性。

    Distinguishing cells in a sample by inactivating extracellular enzyme before releasing intracellular enzyme
    9.
    发明授权
    Distinguishing cells in a sample by inactivating extracellular enzyme before releasing intracellular enzyme 有权
    通过在释放细胞内酶之前使细胞外酶失活来区分样品中的细胞

    公开(公告)号：US08349583B2

    公开(公告)日：2013-01-08

    申请号：US11919832

    申请日：2006-05-03

    申请人： Marc Green ,  Robert Eisenthal ,  Janet Eisenthal, legal representative

    发明人： Marc Green ,  Robert Eisenthal

    IPC分类号： C12Q1/22

    CPC分类号： C12Q1/485 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/66 ,  G01N2333/912 ,  G01N2333/976

    摘要： A method for detecting the absence or presence of cells of interest in a liquid sample is provided. The method comprises providing a sample suspected of containing cells of interest that contain an intracellular enzyme with a measurable activity. The sample further comprises an extracellular medium that also includes an extracellular enzyme with the measurable activity. The method further comprises the steps of treating the liquid sample with a reagent that inactivates the measurable activity in the extracellular medium but does not inactivate the measurable activity in the cells of interest, lysing the cells of interest to release the intracellular enzyme, and measuring the measurable activity. Thus, the measurable activity of the intracellular enzyme can be measured without interference from the extracellular enzyme. The invention is particularly useful for treatment of bacterially-infected blood using a detection assay based on adenylate kinase activity.

    摘要翻译： 提供了用于检测液体样品中感兴趣的细胞的存在或不存在的方法。 该方法包括提供怀疑含有具有可测量活性的细胞内酶的感兴趣细胞的样品。 样品还包含细胞外培养基，其还包含具有可测量活性的细胞外酶。 该方法还包括以下步骤：用试剂灭活液体样品，所述试剂使细胞外培养基中的可测量活性失活，但不灭活感兴趣细胞中的可测量活性，裂解感兴趣的细胞以释放细胞内酶，并测量 可测量的活动。 因此，可以测量细胞内酶的可测量的活性而不受细胞外酶的干扰。 使用基于腺苷酸激酶活性的检测测定法，本发明特别可用于治疗细菌感染的血液。

    HIGH-MOLECULAR-WEIGHT ADIPONECTIN MEASUREMENT METHOD
    10.
    发明申请
    HIGH-MOLECULAR-WEIGHT ADIPONECTIN MEASUREMENT METHOD 审中-公开
    高分子量ADIPONECTIN测量方法

    公开(公告)号：US20120322094A1

    公开(公告)日：2012-12-20

    申请号：US13596695

    申请日：2012-08-28

    申请人： Hiroyuki EBINUMA

    发明人： Hiroyuki EBINUMA

    IPC分类号： C12Q1/37

    CPC分类号： G01N33/74 ,  G01N2333/976

    摘要： Provided is a method of separating and measuring highly active HMW adiponectin in adiponectin multimers. A method of measuring high-molecular-weight adiponectin in a sample, wherein adiponectin multimers are separated by use of a protease and measured immunologically, the method comprising reacting a sample containing adiponectin multimers with chymotrypsin.

    摘要翻译： 提供了在脂联素多聚体中分离和测量高活性HMW脂联素的方法。 一种测量样品中高分子量脂联素的方法，其中通过使用蛋白酶分离脂联素多聚体并进行免疫测定，所述方法包括使含有脂联素多聚体的样品与糜蛋白酶反应。