公开(公告)号：US20210070800A1

公开(公告)日：2021-03-11

申请号：US17100239

申请日：2020-11-20

Applicant: AJINOMOTO CO., INC.

Inventor： Natalia V. STOYNOVA ,  Elena V. SYCHEVA ,  Natalia V. GERASKINA ,  Elena V. MATROSOVA ,  Sergey V. SMIRNOV ,  Ayako SATO ,  Eri HIGASHIURA ,  Misato OKAMOTO ,  Takayuki ITO ,  Erika WATANABE ,  Yuki ODA ,  Uno TAGAMI ,  Tatsuki KASHIWAGI ,  Masayuki SUGIKI

IPC: C07K5/093

Abstract: A method for producing γ-Glu-Val-Gly is described, wherein the method includes the steps of cultivating a γ-Glu-Val-Gly-producing bacterium belonging to the family Enterobacteriaceae in a culture medium so that the γ-Glu-Val-Gly accumulates in the culture medium or the cells of the bacterium, or both, and collecting the γ-Glu-Val-Gly from the culture medium or the cells of the bacterium, or both. The bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity.

公开(公告)号：US20190264191A1

公开(公告)日：2019-08-29

申请号：US16287459

申请日：2019-02-27

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SATO ,  Eri HIGASHIURA ,  Misato OKAMOTO ,  Takayuki ITO ,  Erika WATANABE ,  Uno TAGAMI ,  Yuki ODA ,  Tatsuki KASHIWAGI ,  Masayuki SUGIKI

IPC: C12N9/00 ,  C12P21/02

Abstract: A mutant glutathione synthetase (GSHB) suitable for generating γ-Glu-Val-Gly, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val-Gly is produced by using a mutant GSHB having a mutation at such a position as V7, N13, I14, N15, K17, F22, F95, M165, N199, Y200, P202, I274, T285, and P287.