公开(公告)号：US20190264191A1

公开(公告)日：2019-08-29

申请号：US16287459

申请日：2019-02-27

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SATO ,  Eri HIGASHIURA ,  Misato OKAMOTO ,  Takayuki ITO ,  Erika WATANABE ,  Uno TAGAMI ,  Yuki ODA ,  Tatsuki KASHIWAGI ,  Masayuki SUGIKI

IPC: C12N9/00 ,  C12P21/02

Abstract: A mutant glutathione synthetase (GSHB) suitable for generating γ-Glu-Val-Gly, and a method for producing γ-Glu-Val-Gly using the same are provided. γ-Glu-Val-Gly is produced by using a mutant GSHB having a mutation at such a position as V7, N13, I14, N15, K17, F22, F95, M165, N199, Y200, P202, I274, T285, and P287.

公开(公告)号：US20230399673A1

公开(公告)日：2023-12-14

申请号：US18454052

申请日：2023-08-22

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SASAHARA ,  Takayuki ITO ,  Hiroyuki NOZAKI

IPC: C12P21/02 ,  C12N1/20 ,  C07K5/083 ,  C07K5/093 ,  C12N9/00

CPC classification number: C12P21/02 ,  C12N1/205 ,  C07K5/0806 ,  C07K5/0819 ,  C12N9/93 ,  C12N15/09

Abstract: A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, such as Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDK) is reduced as an expression host, γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.

公开(公告)号：US20210070800A1

公开(公告)日：2021-03-11

申请号：US17100239

申请日：2020-11-20

Applicant: AJINOMOTO CO., INC.

Inventor： Natalia V. STOYNOVA ,  Elena V. SYCHEVA ,  Natalia V. GERASKINA ,  Elena V. MATROSOVA ,  Sergey V. SMIRNOV ,  Ayako SATO ,  Eri HIGASHIURA ,  Misato OKAMOTO ,  Takayuki ITO ,  Erika WATANABE ,  Yuki ODA ,  Uno TAGAMI ,  Tatsuki KASHIWAGI ,  Masayuki SUGIKI

IPC: C07K5/093

Abstract: A method for producing γ-Glu-Val-Gly is described, wherein the method includes the steps of cultivating a γ-Glu-Val-Gly-producing bacterium belonging to the family Enterobacteriaceae in a culture medium so that the γ-Glu-Val-Gly accumulates in the culture medium or the cells of the bacterium, or both, and collecting the γ-Glu-Val-Gly from the culture medium or the cells of the bacterium, or both. The bacterium has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity.

公开(公告)号：US20170067085A1

公开(公告)日：2017-03-09

申请号：US15352928

申请日：2016-11-16

Applicant: AJINOMOTO CO., INC.

Inventor： Hiroaki NISHIUCHI ,  Misato MORITA ,  Wataru HOSHINO ,  Junko YAMAZAKI ,  Takayuki ITO ,  Kazuo YAMAGISHI

IPC: C12P13/14 ,  C12P13/04 ,  A23L27/24 ,  A23L33/145 ,  A23L31/15 ,  A23L27/22

CPC classification number: A23L31/15 ,  A23L5/00 ,  A23L27/22 ,  A23L27/24 ,  A23L31/00 ,  A23L33/14 ,  A23L33/145 ,  A23V2002/00 ,  C12N1/18 ,  C12N9/1096 ,  C12N9/93 ,  C12N15/81 ,  C12P13/04 ,  C12P13/14 ,  C12Y203/02002 ,  C12Y603/02002 ,  C12Y603/02003

Abstract: A yeast extract containing 0.2% or more of γ-Glu-Abu based on dry weight of the yeast extract is produced by culturing a yeast, such as Saccharomyces cervisiae or Candida utilis, in a medium containing a compound selected from Abu (L-2-aminobutyric acid) and γ-Glu-Abu (L-γ-glutamyl-L-2-aminobutyric acid), and preparing a yeast extract from the obtained cells.

Abstract translation: 通过在含有选自Abu（L-2）的化合物的培养基中培养酵母，例如酵母提取物的干重，含有0.2％以上的γ-Glu-Abu的酵母提取物， - 氨基丁酸）和γ-Glu-Abu（L-γ-谷氨酰基-L-2-氨基丁酸），并从得到的细胞中制备酵母提取物。

公开(公告)号：US20180195103A1

公开(公告)日：2018-07-12

申请号：US15911731

申请日：2018-03-05

Applicant: AJINOMOTO CO., INC.

Inventor： Ayako SASAHARA ,  Takayuki ITO ,  Hiroyuki NOZAKI

IPC: C12P21/02 ,  C12R1/19 ,  C12N9/00 ,  C07K5/083 ,  C07K5/093

CPC classification number: C12P21/02 ,  C07K5/0806 ,  C07K5/0819 ,  C12N9/93 ,  C12N15/09 ,  C12R1/19 ,  C12Y603/02002 ,  C12Y603/02003

Abstract: A microorganism useful as an expression host for γ-Glu-Val synthetase and a method for producing γ-Glu-Val-Gly using γ-Glu-Val synthetase expressed in the microorganism are provided. By using γ-Glu-Val synthetase expressed in a bacterium, such as Escherichia bacteria, modified so that the activity of a protein encoded by a ybdK gene (YBDK) is reduced as an expression host, γ-Glu-Val-Gly is produced from Glu, Val, and Gly as raw materials.