公开(公告)号：US20210147922A1

公开(公告)日：2021-05-20

申请号：US17047456

申请日：2019-04-18

申请人： ALTIUS INSTITUTE FOR BIOMEDICAL SCIENCES

发明人： Fyodor Urnov ,  John A. Stamatoyannopoulos ,  Vivek Nandakumar ,  Pavel Zrazhevskiy ,  Shreeram Akilesh

IPC分类号： C12Q1/6841 ,  C12N15/10

摘要： The present disclosure provides methods and compositions for image based analysis and quantification of a protein load from protein (e.g., p53BP1) accumulation, induced by a cellular perturbation, such as administration of a genome editing tool comprising a DNA binding domain and a nuclease domain, a gene repressor, or a gene activator.

公开(公告)号：US20220397526A1

公开(公告)日：2022-12-15

申请号：US17736684

申请日：2022-05-04

申请人： Altius Institute for Biomedical Sciences

发明人： John A. Stamatoyannopoulos ,  Shreeram Akilesh ,  Vivek Nandakumar

IPC分类号： G01N21/64 ,  C12Q1/6841 ,  C40B40/06 ,  G01N21/78 ,  G01N33/574 ,  G01N33/58 ,  G01N33/569 ,  A01N1/02 ,  C12N5/078 ,  C12N5/09 ,  C12P19/34 ,  G01N1/30 ,  C12Q1/6837

摘要： Disclosed herein are methods of detecting a target nucleic acid sequence, determining the localization of the target nucleic acid sequence, and/or quantifying the number of target nucleic acid sequences in a cell. This method may be used on small target nucleic acid sequences, and may be referred to as Nano-FISH.

公开(公告)号：US11353400B2

公开(公告)日：2022-06-07

申请号：US16319157

申请日：2017-07-19

申请人： Altius Institute for Biomedical Sciences

发明人： John A. Stamatoyannopoulos ,  Shreeram Akilesh ,  Vivek Nandakumar

IPC分类号： G01N21/64 ,  C12Q1/6841 ,  C40B40/06 ,  G01N21/78 ,  G01N33/574 ,  G01N33/58 ,  G01N33/569 ,  A01N1/02 ,  C12N5/078 ,  C12N5/09 ,  C12P19/34 ,  G01N1/30 ,  C12Q1/6837 ,  C40B30/04 ,  G01N15/00

摘要： Disclosed herein are methods of detecting a target nucleic acid sequence, determining the localization of the target nucleic acid sequence, and/or quantifying the number of target nucleic acid sequences in a cell. This method may be used on small target nucleic acid sequences, and may be referred to as Nano-FISH.