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公开(公告)号：US20220397526A1
公开(公告)日：2022-12-15
申请号：US17736684
申请日：2022-05-04
IPC分类号： G01N21/64 , C12Q1/6841 , C40B40/06 , G01N21/78 , G01N33/574 , G01N33/58 , G01N33/569 , A01N1/02 , C12N5/078 , C12N5/09 , C12P19/34 , G01N1/30 , C12Q1/6837
摘要： Disclosed herein are methods of detecting a target nucleic acid sequence, determining the localization of the target nucleic acid sequence, and/or quantifying the number of target nucleic acid sequences in a cell. This method may be used on small target nucleic acid sequences, and may be referred to as Nano-FISH.
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公开(公告)号：US11353400B2
公开(公告)日：2022-06-07
申请号：US16319157
申请日：2017-07-19
IPC分类号： G01N21/64 , C12Q1/6841 , C40B40/06 , G01N21/78 , G01N33/574 , G01N33/58 , G01N33/569 , A01N1/02 , C12N5/078 , C12N5/09 , C12P19/34 , G01N1/30 , C12Q1/6837 , C40B30/04 , G01N15/00
摘要： Disclosed herein are methods of detecting a target nucleic acid sequence, determining the localization of the target nucleic acid sequence, and/or quantifying the number of target nucleic acid sequences in a cell. This method may be used on small target nucleic acid sequences, and may be referred to as Nano-FISH.
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公开(公告)号：US20210310058A1
公开(公告)日：2021-10-07
申请号：US16631632
申请日：2018-07-19
发明人： Shreeram AKILESH , John A. STAMATOYANNOPOULOS , Alessandra SULLIVAN , William KERWIN , Tobias RAGOCZY , Pavel ZRAZHEVSKIY
IPC分类号： C12Q1/6841 , C12Q1/6876
摘要： Disclosed herein are methods of detecting a target viral nucleic acid sequence, determining the localization of the target viral nucleic acid sequence, and/or quantifying the number of target viral nucleic acid sequences in a cell. This method may be used on small target nucleic acid sequences, and may be referred to as Nano-FISH or viral Nano-FISH.
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公开(公告)号：US20200167914A1
公开(公告)日：2020-05-28
申请号：US16630090
申请日：2018-07-19
发明人： John A. STAMATOYANNOPOULOS , Shreeram AKILESH , Alexander MURATOV , Wouter MEULEMAN , William KERWIN
摘要： Disclosed herein are methods of utilizing machine learning methods to analyze microscope images of populations of cells.
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公开(公告)号：US20230257722A1
公开(公告)日：2023-08-17
申请号：US17253863
申请日：2019-06-26
CPC分类号： C12N9/22 , C07K19/00 , C12N15/52 , C07K2319/81
摘要： DNA cleaving enzymes are disclosed. The DNA cleaving enzymes are fused to a heterologous DNA binding domain that is designed to bind to a target nucleic acid sequence. Nucleic acids and expression cassettes encoding the DNA cleaving enzymes are also provided. Methods for genome editing using the DNA cleaving enzymes, fusion proteins, and compositions thereof are disclosed herein.
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6.发明公开公开(公告)号：US20230227513A1
公开(公告)日：2023-07-20
申请号：US17764497
申请日：2020-10-14
IPC分类号： C07K14/47 , C07K14/805 , A61P7/06
CPC分类号： C07K14/4702 , C07K14/805 , A61P7/06 , C07K2319/80 , A61K38/00
摘要： The present disclosure provides methods and compositions for modulating expression of a target gene in a cell by reducing binding of an endogenous transcription factor to a regulatory sequence of the target gene. The method includes introducing into the cell a DNA binding polypeptide (DBF) that binds a sequence in regulatory region of a target gene bound by a transcription factor (TF), thereby displacing the TF and modulating expression of the target gene. The DBF may be designed to bind a sequence comprising the binding site for the TF and additional nucleotides present on one or both sides of the sequence. Accordingly, the DBF specifically binds to binding site for the TF in the target gene but not in other genes that are also regulated by binding of the TF but do not include the nucleotides present on one or both sides of the sequence.
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公开(公告)号：US20220010366A1
公开(公告)日：2022-01-13
申请号：US17293869
申请日：2019-11-18
IPC分类号： C12Q1/6816
摘要： Provided herein are methods and compositions for use in detecting on-target and/or off-target cleavage of genomic DNA in a cell by a nuclease. The on-target and off-target cleavage is detected by using oligonucleotides that bind to the single-stranded 3′-overhang created at the cleavage site. The nuclease may be an engineered nuclease comprising a nucleic acid binding domain that binds to a target nucleic acid of interest.
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公开(公告)号：US20190234874A1
公开(公告)日：2019-08-01
申请号：US16319099
申请日：2017-07-19
CPC分类号： G01N21/64 , A01N1/0221 , C12N5/0634 , C12N5/0694 , C12P19/34 , C12Q1/6841 , C40B30/04 , C40B40/06 , G01N1/30 , G01N21/6458 , G01N21/78 , G01N33/56966 , G01N33/574 , G01N33/582 , G01N2015/0038 , C12Q2521/301 , C12Q2563/107
摘要： Disclosed herein are methods of detecting a regulatory element, determining the localization of a regulatory element, and/or measuring the activity of a regulatory element.
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公开(公告)号：US11900600B2
公开(公告)日：2024-02-13
申请号：US18045342
申请日：2022-10-10
发明人： John A. Stamatoyannopoulos , Shreeram Akilesh , Alexander Muratov , Wouter Meuleman , William Kerwin
CPC分类号： G06T7/0012 , G06N20/00 , G16B40/20 , G16B40/30 , G06T2207/20081 , G06T2207/20084 , G06T2207/30024
摘要： Disclosed herein are methods of utilizing machine learning methods to analyze microscope images of populations of cells.
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公开(公告)号：US20220372089A1
公开(公告)日：2022-11-24
申请号：US17605995
申请日：2020-04-23
摘要： The present disclosure provides genome engineering proteins, e.g., nucleic acid binding domains and/or functional domains that have a net positive charge and are cell permeable and can be introduced into the cells without the use of a carrier such as micelles, vesicles, liposomes, and the like.
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