公开(公告)号：US20230204597A1

公开(公告)日：2023-06-29

申请号：US17910653

申请日：2021-03-19

Applicant: AMGEN INC.

Inventor： Zhongqi Zhang ,  Bhavana Shah

IPC: G01N33/68 ,  C12Q1/37

CPC classification number: G01N33/6863 ,  C12Q1/37 ,  G01N2333/535

Abstract: The present disclosure provides materials and methods for determining the presence of an N-terminal modification on a therapeutic protein, and/or the efficiency of N-terminal modification, such as PEGylation, at the N-terminus of a therapeutic protein such as Filgrastim (wherein the PEGylated version is therefore Pegfilgrastim).