公开(公告)号：US11254963B2

公开(公告)日：2022-02-22

申请号：US16676340

申请日：2019-11-06

Applicant: Amgen Inc.

Inventor： Sohye Kang ,  Chung Huang, Jr. ,  Hedieh Barkhordarian ,  Pavel Bondarenko ,  Zhongqi Zhang

IPC: C12P21/00 ,  C07K16/00 ,  C12N1/38 ,  C07K14/52 ,  C12N5/071

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

公开(公告)号：US20230204597A1

公开(公告)日：2023-06-29

申请号：US17910653

申请日：2021-03-19

Applicant: AMGEN INC.

Inventor： Zhongqi Zhang ,  Bhavana Shah

IPC: G01N33/68 ,  C12Q1/37

CPC classification number: G01N33/6863 ,  C12Q1/37 ,  G01N2333/535

Abstract: The present disclosure provides materials and methods for determining the presence of an N-terminal modification on a therapeutic protein, and/or the efficiency of N-terminal modification, such as PEGylation, at the N-terminus of a therapeutic protein such as Filgrastim (wherein the PEGylated version is therefore Pegfilgrastim).

公开(公告)号：US10513723B2

公开(公告)日：2019-12-24

申请号：US15111470

申请日：2014-12-09

Applicant: AMGEN INC.

Inventor： Sohye Kang ,  Chung Huang, Jr. ,  Hedieh Barkhordarian ,  Pavel Bondarenko ,  Zhongqi Zhang

IPC: C12P21/00 ,  C12N5/071 ,  C07K14/52 ,  C07K16/00 ,  C12N1/38

Abstract: The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

公开(公告)号：US11854779B2

公开(公告)日：2023-12-26

申请号：US16972742

申请日：2019-06-06

Applicant: AMGEN INC.

Inventor： Zhongqi Zhang

IPC: H01J49/00 ,  G01N30/86 ,  G01N33/68

CPC classification number: H01J49/0009 ,  G01N30/8665 ,  G01N30/8696 ,  H01J49/0036 ,  G01N30/86 ,  G01N33/68

Abstract: Systems and methods are described for reducing lab-to-lab and/or instrument-to-instrument variability of Multi-Attribute Methods (MAM) analyses via run-time signal intensity calibration. In various aspects, multiple MAM-based instruments each have detectors and different instrument conditions defined by different instrument models or sets of settings. Each MAM-based instrument receives respective samples and a reference standard as a calibrant. Each MAM-based instrument detects, via its detector, sample isoforms of its respective sample and reference standard isoforms of the reference standard. The MAM-based instruments are associated with processor(s) that determine, via respective MAM iterations, correction factors and sample abundance values corresponding to the sample isoforms. The correction factors are based on the reference standard, and the sample abundance values are based on the correction factors. A variance value of the sample abundance values may be reduced based on correction factors of each of the MAM-based instruments.