公开(公告)号：US20210395793A1

公开(公告)日：2021-12-23

申请号：US17464115

申请日：2021-09-01

Applicant: Amgen Inc.

Inventor： Sandhya PANDE ,  Mirna MUJACIC

IPC: C12P21/00

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the addition of cell-cycle inhibitors to growing cell cultures.

公开(公告)号：US20160281124A1

公开(公告)日：2016-09-29

申请号：US15033559

申请日：2014-10-30

Applicant: AMGEN INC.

Inventor： Sandhya PANDE ,  Mirna MUJACIC

IPC: C12P21/00

CPC classification number: C12P21/005

Abstract: Methods of modulating high mannose glycoform content of a protein in a cell culture by contacting the cells expressing the protein of interest with monesin are provided.

Abstract translation: 提供了通过使表达目标蛋白质的细胞与葡萄糖蛋白接触来调节细胞培养物中蛋白质的高甘露糖糖型含量的方法。

公开(公告)号：US20150376221A1

公开(公告)日：2015-12-31

申请号：US14767737

申请日：2014-02-20

Applicant: AMGEN INC.

Inventor： John Gordon Allen ,  Christopher H. FOTSCH ,  Michael J. FROHN ,  John Douglas MCCARTER ,  Mirna MUJACIC ,  Alexander J. PICKRELL

IPC: C07H7/02 ,  C12N5/073 ,  C07K16/28

CPC classification number: C07H7/02 ,  C07D309/10 ,  C07D309/14 ,  C07K16/2875 ,  C07K2317/14 ,  C07K2317/41 ,  C12N5/0603 ,  C12N2501/999 ,  C12N2510/00

Abstract: Compounds of Formula (I) are useful as modulators of glycosylation. Compounds of Formula (I) have the following structure: (I) and the definitions of the other variables are provided herein.

Abstract translation: 式（I）的化合物可用作糖基化的调节剂。 式（I）的化合物具有以下结构：（I），本文提供其它变量的定义。