公开(公告)号：US20220276157A1

公开(公告)日：2022-09-01

申请号：US17632886

申请日：2020-08-06

Applicant: AMGEN INC

Inventor： Shang Zeng ,  Gang Xue

IPC: G01N21/33 ,  G01N33/68 ,  G01J3/28

Abstract: ultraviolet (UV) based imaging method for determining protein concentrations of unknown protein samples based on automated multi-wavelength calibration. In various embodiments, a processor receives each of a standard set of wavelength data and an unknown set of wavelength data as recorded by a detector. Each standard set of wavelength data and unknown set of wavelength data defines a series of absorbance-to-wavelength value pairs across a first range of wavelengths selected from a range of a single-wavelength light beams of a UV spectra. The processor generates a multi-wavelength calibration model based on each of a first series of first absorbance-to-wavelength value pairs of the standard set of wavelength data. The processor implements the multi-wavelength calibration model to determine, for each unknown protein sample of the given unknown protein samples, a plurality of protein concentration values.

公开(公告)号：US12181404B2

公开(公告)日：2024-12-31

申请号：US17632886

申请日：2020-08-06

Applicant: AMGEN INC.

Inventor： Shang Zeng ,  Gang Xue

IPC: G01N21/33 ,  G01J3/28 ,  G01N33/68

Abstract: ultraviolet (UV) based imaging method for determining protein concentrations of unknown protein samples based on automated multi-wavelength calibration. In various embodiments, a processor receives each of a standard set of wavelength data and an unknown set of wavelength data as recorded by a detector. Each standard set of wavelength data and unknown set of wavelength data defines a series of absorbance-to-wavelength value pairs across a first range of wavelengths selected from a range of a single-wavelength light beams of a UV spectra. The processor generates a multi-wavelength calibration model based on each of a first series of first absorbance-to-wavelength value pairs of the standard set of wavelength data. The processor implements the multi-wavelength calibration model to determine, for each unknown protein sample of the given unknown protein samples, a plurality of protein concentration values.