公开(公告)号：US10329564B2

公开(公告)日：2019-06-25

申请号：US15696172

申请日：2017-09-05

申请人： Applied Biosystems, LLC

发明人： Nitin Puri ,  Irudaya Charles ,  Susan Maylin Magdaleno ,  Alexander Vlassov ,  Christopher Burnett

IPC分类号： C12N15/113 ,  C12N15/11

摘要： Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.

公开(公告)号：US10900038B2

公开(公告)日：2021-01-26

申请号：US16409689

申请日：2019-05-10

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Nitin Puri ,  Irudaya Charles ,  Susan Maylin Magdaleno ,  Alexander Vlassov ,  Christopher Burnett

IPC分类号： C12N15/113 ,  C12N15/11

摘要： Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.

公开(公告)号：US09771583B2

公开(公告)日：2017-09-26

申请号：US15015912

申请日：2016-02-04

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Nitin Puri ,  Irudaya Charles ,  Susan Magdaleno ,  Alexander Vlassov ,  Christopher Burnett

IPC分类号： C12N15/113 ,  C12N15/11

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N15/1137 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2310/319 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2310/343 ,  C12N2320/53 ,  C12N2310/3521

摘要： Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.

公开(公告)号：US09273312B2

公开(公告)日：2016-03-01

申请号：US13975136

申请日：2013-08-23

申请人： Applied Biosystems, LLC

发明人： Nitin Puri ,  Irudaya Charles ,  Susan Magdaleno ,  Alexander Vlassov ,  Christopher Burnett

IPC分类号： C12N15/113 ,  C12N15/11

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N15/1137 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2310/319 ,  C12N2310/321 ,  C12N2310/3231 ,  C12N2310/343 ,  C12N2320/53 ,  C12N2310/3521

摘要： Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.

摘要翻译： 提供了具有修饰的核苷酸的修饰形式用于siRNA。 具有修饰形式的短干扰RNA和本文提供的经修饰的核苷酸减少RNA干扰内源基因的脱靶效应。 证明进一步修饰格式化的siRNA被稳定到富含核酸酶的环境中。 意外的是，增加或维持链偏倚，同时必需保持内源性RNA干扰的效力，不足以减少细胞生物学测定中的脱靶效应。