公开(公告)号：US20160024566A1

公开(公告)日：2016-01-28

申请号：US14876711

申请日：2015-10-06

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Shoulian DONG ,  Junko Stevens ,  Danny Lee

IPC分类号： C12Q1/68 ,  C12N15/115

CPC分类号： C12Q1/6846 ,  C12N15/115 ,  C12N2310/16 ,  C12P19/34 ,  C12Q1/6848 ,  G01N21/6486 ,  C12Q2549/125 ,  C12Q2527/127 ,  C12Q2527/107

摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

摘要翻译： 本教导涉及用于扩增靶核酸的组合物，方法和试剂盒，同时减少非特异性荧光和不需要的扩增产物，有时称为二级扩增产物或杂种副产物。 本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。 还提供了包含与酶相关的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。 公开了减少不期望的扩增产物同时扩增靶核酸的方法，还有用于降低非特异性荧光的方法。 还提供了用于加速某些公开方法的执行的套件。

公开(公告)号：US20140004515A1

公开(公告)日：2014-01-02

申请号：US13902742

申请日：2013-05-24

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Shoulian DONG ,  Junko Stevens ,  Danny Lee

IPC分类号： G01N21/64

CPC分类号： C12Q1/6846 ,  C12N15/115 ,  C12N2310/16 ,  C12P19/34 ,  C12Q1/6848 ,  G01N21/6486 ,  C12Q2549/125 ,  C12Q2527/127 ,  C12Q2527/107

摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

摘要翻译： 本教导涉及用于扩增靶核酸的组合物，方法和试剂盒，同时减少非特异性荧光和不需要的扩增产物，有时称为二级扩增产物或假副产物。 本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。 还提供了包含与酶缔合的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。 公开了减少不期望的扩增产物同时扩增靶核酸的方法，还有用于降低非特异性荧光的方法。 还提供了用于加速某些公开方法的执行的套件。