公开(公告)号：US20160024566A1

公开(公告)日：2016-01-28

申请号：US14876711

申请日：2015-10-06

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Shoulian DONG ,  Junko Stevens ,  Danny Lee

IPC分类号： C12Q1/68 ,  C12N15/115

CPC分类号： C12Q1/6846 ,  C12N15/115 ,  C12N2310/16 ,  C12P19/34 ,  C12Q1/6848 ,  G01N21/6486 ,  C12Q2549/125 ,  C12Q2527/127 ,  C12Q2527/107

摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

摘要翻译： 本教导涉及用于扩增靶核酸的组合物，方法和试剂盒，同时减少非特异性荧光和不需要的扩增产物，有时称为二级扩增产物或杂种副产物。 本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。 还提供了包含与酶相关的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。 公开了减少不期望的扩增产物同时扩增靶核酸的方法，还有用于降低非特异性荧光的方法。 还提供了用于加速某些公开方法的执行的套件。

公开(公告)号：US10689692B2

公开(公告)日：2020-06-23

申请号：US15256827

申请日：2016-09-06

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Ryan T. Koehler ,  Kenneth J. Livak ,  Junko Stevens ,  Francisco M. De La Vega ,  Michael Rhodes ,  Laurent R. Bellon ,  Dawn Madden ,  Dennis A. Gilbert ,  Yu N. Wang ,  Eugene G. Spier ,  Xiaoqing You ,  Lily Xu ,  Jeremy Heil ,  Emily S. Winn-Deen ,  Ivy McMullen ,  Susan Eddins ,  Leila G. Smith

IPC分类号： C12Q1/6858 ,  G16B30/00 ,  G16B35/00 ,  G16B50/00 ,  G16C20/60 ,  G06Q30/06 ,  G06Q50/22 ,  B01L3/00 ,  G01N35/00 ,  G16B25/00 ,  G16B45/00

摘要： Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.

公开(公告)号：US20140329238A1

公开(公告)日：2014-11-06

申请号：US14225332

申请日：2014-03-25

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Kenneth LIVAK ,  Junko Stevens ,  Katherine Lazaruk ,  Janet Ziegle ,  Lily Wong

IPC分类号： G06F19/18 ,  C12Q1/68

CPC分类号： G16B20/00 ,  C12Q1/6827 ,  C12Q1/6851 ,  C12Q2600/156 ,  C12Q2563/107 ,  C12Q2545/101 ,  C12Q2565/101 ,  C12Q2563/173 ,  C12Q2565/1015

摘要： Methods of detecting a candidate genetic anomaly such as a candidate duplication in a genome are disclosed. The methods comprise quantifying fluorogenic assays for alleles of a genetic locus from a plurality of individual genomes, identifying ranges of fluorescent intensities indicative of individual genomes homozygous for a first allele, homozygous for a second allele, or heterozygous for both alleles, and identifying individual genomes in which the fluorescence intensities are outside the range of intensities indicative of homozygosity or heterozygosity for the genetic locus.

摘要翻译： 公开了检测候选遗传异常（例如基因组中候选重复）的方法。 所述方法包括定量来自多个单个基因组的遗传基因座的等位基因的荧光测定，鉴定指示第一等位基因纯合的单个基因组的荧光强度的范围，对于第二等位基因纯合，或对于两个等位基因是杂合的，并鉴定个体基因组 其中荧光强度在表示遗传基因座的纯合性或杂合性的强度范围之外。

公开(公告)号：US20140004515A1

公开(公告)日：2014-01-02

申请号：US13902742

申请日：2013-05-24

申请人： APPLIED BIOSYSTEMS, LLC

发明人： Shoulian DONG ,  Junko Stevens ,  Danny Lee

IPC分类号： G01N21/64

CPC分类号： C12Q1/6846 ,  C12N15/115 ,  C12N2310/16 ,  C12P19/34 ,  C12Q1/6848 ,  G01N21/6486 ,  C12Q2549/125 ,  C12Q2527/127 ,  C12Q2527/107

摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

摘要翻译： 本教导涉及用于扩增靶核酸的组合物，方法和试剂盒，同时减少非特异性荧光和不需要的扩增产物，有时称为二级扩增产物或假副产物。 本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。 还提供了包含与酶缔合的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。 公开了减少不期望的扩增产物同时扩增靶核酸的方法，还有用于降低非特异性荧光的方法。 还提供了用于加速某些公开方法的执行的套件。