公开(公告)号：US20220316002A1

公开(公告)日：2022-10-06

申请号：US17620566

申请日：2020-06-19

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Stuart LINDSAY ,  Bintian ZHANG ,  Hanqing DENG

IPC: C12Q1/6869 ,  C12Q1/00 ,  G01N27/447

Abstract: Disclosed are devices, systems and methods for direct measurement of polymerase activity. In one example, a device includes at least a first electrode and a second electrode, the first and second electrode being separated by a gap; and a polymerase with two attachment sites, one for attaching to the first electrode and a second for attaching to the second electrode, wherein the two attachment sites are separated by a distance of at least about 1 nm and the distance does not significantly change with conformational changes of the polymerase.