公开(公告)号：US20170204066A1

公开(公告)日：2017-07-20

申请号：US15417903

申请日：2017-01-27

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart LINDSAY ,  Peiming ZHANG

IPC: C07D235/24 ,  G01N33/487 ,  G01N27/447 ,  C12Q1/68

CPC classification number: C07D235/24 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/631

Abstract: The present invention is directed to systems, devices and methods for identifying biopolymers, such as strands of DNA, as they pass through a constriction such as a carbon nanotube nanopore. More particularly, the invention is directed to such systems, devices and methods in which a newly translocated portion of the biopolymer forms a temporary electrical circuit between the nanotube nanopore and a second electrode, which may also be a nanotube. Further, the invention is directed to such systems, devices and methods in which the constriction is provided with a functionalized unit which, together with a newly translocated portion of the biopolymer, forms a temporary electrical circuit that can be used to characterize that portion of the biopolymer.

公开(公告)号：US20170003245A1

公开(公告)日：2017-01-05

申请号：US15203383

申请日：2016-07-06

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart LINDSAY ,  Peiming ZHANG

IPC: G01N27/327 ,  G01N33/487 ,  C12Q1/68 ,  G01N27/02

CPC classification number: G01N27/3278 ,  C12Q1/6869 ,  G01N27/028 ,  G01N27/3276 ,  G01N33/48721 ,  C12Q2565/631

Abstract: An system for recognition of a translocating polymetric target molecule includes a device having at least one constriction that is sized to permit translocation of only a single copy of the molecule. A pair of spaced apart sensing electrodes border the constriction, which may be a nonopore. The first electrode is connected to a first affinity element and the second electrode is connected to a second affinity element. Each affinity element may connected to its corresponding electrode via one or more intermediary compounds, such as a linker molecule and/or an electrode attachment molecule. The first and second affinity elements are configured to temporarily form hydrogen bonds with first and second portions of the target molecule as the latter passes through the constriction. During translocation, the electrodes affinity elements and first and second portions of the target molecule complete an electrical circuit and allow a measurable electrical current to pass between the first and second electrodes. The time-varying nature of this electrical current, and the specific affinity elements employed, allow one to characterize the target molecule.

Abstract translation: 用于识别易位多分子靶分子的系统包括具有至少一个收缩部的装置，其尺寸被确定为允许仅分解单个拷贝的易位。 一对间隔开的感应电极与收缩部分相邻，其可以是非孔。 第一电极连接到第一亲和元件，第二电极连接到第二亲和元件。 每个亲和元件可以经由一种或多种中间化合物，例如连接体分子和/或电极附着分子连接到其对应的电极。 第一和第二亲和元件配置成与靶分子的第一和第二部分暂时形成氢键，因为它们经过收缩部。 在易位期间，电极亲和元件和目标分子的第一和第二部分完成电路并允许可测量的电流在第一和第二电极之间通过。 该电流的随时间变化特性以及所使用的特异亲和元件允许表征靶分子。

公开(公告)号：US20160258925A1

公开(公告)日：2016-09-08

申请号：US15002828

申请日：2016-01-21

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Brett GYARFAS ,  Stuart LINDSAY ,  Pei PANG

IPC: G01N33/487 ,  G01N27/447 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  G01N27/44791 ,  G03F7/20

Abstract: Embodiments of the disclosure are directed to a device for molecule sensing. In some embodiments, the device includes a first electrode separated from a second electrode by a dielectric layer. The first electrode comprises a large area electrode and the second electrode comprises a small area electrode. At least one opening (e.g., trench) cut or otherwise created into the dielectric layer exposes a tunnel junction therebetween whereby target molecules in solution can bind across the tunnel junction.

Abstract translation: 本公开的实施例涉及用于分子感测的装置。 在一些实施例中，该装置包括通过介电层与第二电极分离的第一电极。 第一电极包括大面积电极，第二电极包括小面积电极。 在电介质层中切割或以其他方式制造的至少一个开口（例如，沟槽）暴露出其间的隧道结，其中溶液中的目标分子可以跨过隧道结结合。

公开(公告)号：US20220316002A1

公开(公告)日：2022-10-06

申请号：US17620566

申请日：2020-06-19

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Stuart LINDSAY ,  Bintian ZHANG ,  Hanqing DENG

IPC: C12Q1/6869 ,  C12Q1/00 ,  G01N27/447

Abstract: Disclosed are devices, systems and methods for direct measurement of polymerase activity. In one example, a device includes at least a first electrode and a second electrode, the first and second electrode being separated by a gap; and a polymerase with two attachment sites, one for attaching to the first electrode and a second for attaching to the second electrode, wherein the two attachment sites are separated by a distance of at least about 1 nm and the distance does not significantly change with conformational changes of the polymerase.

公开(公告)号：US20190317040A1

公开(公告)日：2019-10-17

申请号：US16389859

申请日：2019-04-19

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart LINDSAY ,  Peiming ZHANG

IPC: G01N27/327 ,  C12Q1/6869 ,  G01N27/02 ,  G01N33/487

Abstract: An system for recognition of a translocating polymeric target molecule includes a device having at least one constriction that is sized to permit translocation of only a single copy of the molecule. A pair of spaced apart sensing electrodes border the constriction, which may be a nanopore. The first electrode is connected to a first affinity element and the second electrode is connected to a second affinity element. Each affinity element may be connected to its corresponding electrode via one or more intermediary compounds, such as a linker molecule and/or an electrode attachment molecule. The first and second affinity elements are configured to temporarily form hydrogen bonds with first and second portions of the target molecule as the latter passes through the constriction. During translocation, the electrodes, affinity elements and first and second portions of the target molecule complete an electrical circuit and allow a measurable electrical current to pass between the first and second electrodes. The time-varying nature of this electrical current, and the specific affinity elements employed, allow one to characterize the target molecule.

公开(公告)号：US20190242885A1

公开(公告)日：2019-08-08

申请号：US16174933

申请日：2018-10-30

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Stuart LINDSAY ,  Peiming ZHANG ,  Pei PANG

IPC: G01N33/543 ,  G01N33/487 ,  B01L3/00

CPC classification number: G01N33/5438 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0861 ,  B01L2300/0887 ,  G01N33/48721

Abstract: A sensing device is provided that includes a tunnel junction created by forming a hole in a layered tunnel junction (for example). A chemically, well-defined surface may be formed by coupling affinity reagents to the electrodes, which, by these means, the surface may be configured to be selective for a particular analyte.

公开(公告)号：US20180209953A1

公开(公告)日：2018-07-26

申请号：US15922436

申请日：2018-03-15

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Stuart LINDSAY ,  Peiming ZHANG

IPC: G01N33/487 ,  G01N33/68 ,  G01N15/12 ,  G01N27/447 ,  C12Q1/37 ,  G01N33/543

CPC classification number: G01N33/48721 ,  C12Q1/37 ,  G01N15/12 ,  G01N27/44704 ,  G01N27/44747 ,  G01N27/44756 ,  G01N33/5438 ,  G01N33/6803 ,  G01N33/6848 ,  G01N2333/952 ,  G01N2570/00 ,  Y10T436/255

Abstract: Embodiments of the present disclosure are directed to methods, systems and devices, for analyzing the molecules. For example, in some embodiments, a system is provided which includes a first volume of conducting fluid, a second volume of conducting fluid, an orifice in communication with said first and second volumes of fluid, and means for applying an electric potential difference between said first and second volumes of fluid. In some such embodiments, a conjugate product is provided which comprises charged polymers each having attached thereto at least one first molecule for analysis, where the product carries a predetermined charge greater than the charge on the first molecule, and upon dissolving a product in the first volume of fluid, the product is directed into the orifice.

公开(公告)号：US20180120286A1

公开(公告)日：2018-05-03

申请号：US15797052

申请日：2017-10-30

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Stuart LINDSAY ,  Peiming ZHANG ,  Yanan ZHAO

IPC: G01N33/487 ,  G01N27/447

Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length L1 and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than 2L1, but less than or equal to the sum of 2L1 and L2.

公开(公告)号：US20160177383A1

公开(公告)日：2016-06-23

申请号：US14971492

申请日：2015-12-16

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Brian Alan ASHCROFT ,  Stuart LINDSAY

IPC: C12Q1/68 ,  G01N27/447

CPC classification number: C12Q1/6869 ,  G01N33/48721

Abstract: A device for electronic sequencing of polymers consisting of a tunnel gap that is self-aligned with a nanochannel.

Abstract translation: 用于电子测序聚合物的装置，其由与纳米通道自对准的隧道间隙组成。

公开(公告)号：US20150142327A1

公开(公告)日：2015-05-21

申请号：US14493961

申请日：2014-09-23

Applicant: ARIZONA BOARD OF REGENTS on behalf of ARIZONA STATE UNIVERSITY

Inventor： Brian Alan ASHCROFT ,  Stuart LINDSAY ,  John SHUMWAY

IPC: G01N27/414 ,  G01N33/68

CPC classification number: G01N33/68 ,  G01N33/48721

Abstract: A method to identify a chemical target trapped in a tunnel junction with a high probability of a correct assignment based on, a single read of the tunnel current signal. The method recognizes and rejects background signals produced in the absence of target molecules, and do so accurately without rejecting useful signals from the target molecules. The identity of signals generated by electron tunneling through an analyte is provided and comprises determining a plurality of characteristics of each signal current spike, generating one or more training signals with a set of analytes, where the analytes may comprise a first analyte, and using the training signals to find one or more boundaries in a space of dimension equal to one or more parameters, wherein the space is partitioned such that a signal from the first analyte of interest is separated from a signal from the second analyte of interest.

Abstract translation: 基于隧道电流信号的单次读取，识别被捕获在隧道结中的化学目标的方法，其具有正确分配的高概率。 该方法识别和排除在不存在目标分子的情况下产生的背景信号，并且准确地进行，而不会排除来自目标分子的有用信号。 提供通过通过分析物的电子穿隧产生的信号的身份，并且包括确定每个信号电流尖峰的多个特征，用一组分析物产生一个或多个训练信号，其中分析物可以包括第一分析物，并且使用 训练信号以在等于一个或多个参数的维度空间中找到一个或多个边界，其中所述空间被分割，使得来自所述第一分析物的信号与来自所述感兴趣的第二分析物的信号分离。