公开(公告)号：US20230296635A1

公开(公告)日：2023-09-21

申请号：US18185808

申请日：2023-03-17

Applicant: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY

Inventor： Shaopeng WANG ,  Zijian WAN ,  Heng ZHU

IPC: G01N33/94 ,  G01N33/543 ,  G01N21/64

CPC classification number: G01N33/94 ,  G01N33/54373 ,  G01N21/6428 ,  G01N2333/035 ,  G01N2500/04 ,  G01N2333/726 ,  G01N2021/6439

Abstract: Provided herein are methods of determining binding kinetics of a ligand. In some embodiments, the methods include contacting the ligand with a first surface of a substrate, which first surface comprises an electrically conductive coating and a population of virions connected to the first surface via one or more linker moieties, wherein viral envelopes of the virions display one or more proteins that bind, or are capable of binding, to the ligand, applying an alternating current electric field to the substrate to induce the virions to oscillate proximal to the first surface of the substrate, and detecting changes in oscillation amplitudes of the virions over a duration. Related virion oscillator array devices, systems and computer readable media are also provided.