公开(公告)号：US20230313169A1

公开(公告)日：2023-10-05

申请号：US17998916

申请日：2021-02-18

申请人： ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD.

发明人： Hao HONG ,  Gage JAMES ,  Yi XIAO ,  Na ZHANG ,  Williams VYASA ,  Yuxia CUI ,  Jiadong ZHAO ,  Yanyan GAO

IPC分类号： C12N11/084 ,  C12N11/04

CPC分类号： C12N11/084 ,  C12N11/04

摘要： Described herein are a PVA membrane immobilized enzyme and a preparation method therefor. The PVA membrane immobilized enzyme includes a PVA porous membrane and an enzyme entrapped on the PVA porous membrane. The PVA porous membrane is a three-dimensional structured PVA porous membrane. The enzyme is any one selected from transaminase, D-lactate dehydrogenase, cyclohexanone monooxygenase, ketoreductase, alkene reductase, nitrilase, ammonia lyase, amino acid dehydrogenase, imine reductase, alcohol dehydrogenase, ammonium formate dehydrogenase, glucose 1-dehydrogenase and mutants thereof. The three-dimensional structured PVA porous membrane is used as a carrier to immobilize an enzyme in an entrapment manner. After entrapping and immobilizing the enzyme in the PVA porous membrane, the enzyme is stable, and cannot be easily leached out in the process of use. The PVA porous membrane is suitable for use in continuous flow biochemical catalysis, and has wide applicability to enzymes.

公开(公告)号：US20230257720A1

公开(公告)日：2023-08-17

申请号：US18001014

申请日：2020-07-08

申请人： ASYMCHEM LIFE SCIENCE (TIANJIN) CO., LTD.

发明人： Hao HONG ,  Gage JAMES ,  Yi XIAO ,  Na ZHANG ,  Fang LIU ,  Zujian WANG ,  Wanming SHI ,  Junjie YAN ,  Mujiao ZHANG

IPC分类号： C12N9/02 ,  C12N15/70 ,  C12P7/02

CPC分类号： C12N9/0004 ,  C12N15/70 ,  C12P7/02 ,  C12Y101/01

摘要： Provided is a ketoreductase mutant and applications thereof. Compared with an amino acid sequence shown in SEQ ID NO: 1, the amino acid sequence of the ketoreductase mutant includes at least one of the following mutation sites: L198P, S96W/I/L/V, M194L or L1526, herein “/” means “or.” The ketoreductase mutant provides high selectivity and high activity with respect to a substrate ketone, and the application of the mutant in a catalytic reduction reaction of a ketone substrate may increase the production efficiency of a chiral alcohol compound corresponding thereto.