公开(公告)号：US12070541B2

公开(公告)日：2024-08-27

申请号：US18323077

申请日：2023-05-24

申请人： Orth Consulting, LLC

发明人： Donald S. Orth ,  Roger W. Orth

IPC分类号： A61M1/34 ,  A61F2/01 ,  A61F2/95 ,  A61K8/66 ,  A61M1/14 ,  A61M1/16 ,  A61M1/26 ,  A61M1/36 ,  B01D63/02 ,  B01D63/06 ,  B01D69/04 ,  B01D69/08 ,  B01J31/00 ,  C07K16/00 ,  C07K16/38 ,  C07K16/40 ,  C12N9/22 ,  C12N11/00 ,  C12N11/04 ,  C12N11/06 ,  C12N11/14 ,  C12N11/16

CPC分类号： A61M1/3489 ,  A61F2/01 ,  A61F2/95 ,  A61K8/66 ,  A61M1/15632 ,  A61M1/1621 ,  A61M1/1678 ,  A61M1/267 ,  A61M1/36 ,  A61M1/3601 ,  A61M1/3618 ,  A61M1/3621 ,  A61M1/3679 ,  A61M1/3687 ,  A61M1/3689 ,  B01D63/02 ,  B01D63/06 ,  B01D69/04 ,  B01D69/043 ,  B01D69/08 ,  B01J31/00 ,  B01J31/003 ,  C07K16/00 ,  C07K16/38 ,  C07K16/40 ,  C12N9/22 ,  C12N11/00 ,  C12N11/04 ,  C12N11/06 ,  C12N11/14 ,  C12N11/16 ,  A61M2202/0445 ,  A61M2205/3303 ,  A61M2205/3368

摘要： A blood treatment method includes the steps of inducing flow of a patient's blood through a blood treatment device inlet and outlet in fluid connection to the circulatory system of the patient. Metastatic deoxyribonucleic acid (DNA) contained within patient blood is destroyed by passing a patient's blood over a bioreactor surface having attached or immobilized deoxyribonuclease 1 (DNase 1) enzyme. The blood treatment device which consists of a bioreactor containing immobilized DNase 1, enables continuous treatment of a patient's blood and increases the effective concentration of DNase 1 in a patient's bloodstream to convert metastasizing cancer DNA in blood into non-oncogenic nucleotide fragments in vivo without adding any chemicals to the blood of the patient.

公开(公告)号：US20240254519A1

公开(公告)日：2024-08-01

申请号：US18628352

申请日：2024-04-05

申请人： Lawrance Livermore National Security, LLC

发明人： Sarah Baker ,  Joshuah K. Stolaroff ,  Congwang Ye

IPC分类号： C12P7/04 ,  B01J31/00 ,  B01J31/06 ,  C12N11/04 ,  C12N11/082 ,  C12N11/084 ,  C12N11/087 ,  C12N11/089 ,  C12N11/096

CPC分类号： C12P7/04 ,  B01J31/003 ,  B01J31/06 ,  C12N11/04 ,  C12N11/082 ,  C12N11/084 ,  C12N11/087 ,  C12N11/089 ,  C12N11/096

摘要： In accordance with one embodiment, a mixture for forming polymer-encapsulated whole cells includes: at least one polymer precursor; at least one initiator; and a plurality of whole cells. In further embodiments, a product includes a structure comprising a plurality of whole cells encapsulated by a polymer network, where polymer(s) of the polymer network are cross-linked. The whole cells may include live whole cells, dried whole cells, and/or reconstituted whole cells, and have a characteristic to convert a chemical reactant to a product, where the chemical reactant is a gas (e.g., C1-C3 carbon gases such as methane, carbon dioxide, ethane, etc.) and the product is a liquid (e.g. methanol, etc.). For example, the whole cells may include organisms such as methanotrophic organisms and/or acetogenic anaerobes.

公开(公告)号：US20240150747A1

公开(公告)日：2024-05-09

申请号：US18281486

申请日：2021-03-17

申请人： The University of Melbourne ,  Grains Research and Development Corporation

发明人： Ruth Elizabeth Haites ,  Helen Jacobe

IPC分类号： C12N11/04 ,  A01N25/04 ,  A01N63/00 ,  A01P9/00 ,  C12N1/10 ,  C12N11/10

CPC分类号： C12N11/04 ,  A01N25/04 ,  A01N63/00 ,  A01P9/00 ,  C12N1/10 ,  C12N11/10

摘要： The present disclosure relates to compositions and methods for inducing encystment of and/or stabilising ciliate cells. In particular, the present disclosure relates to polymers and polymeric solutions, including hydrocolloids, which may be used to induce encystment of trophont ciliate cells to form encysted ciliate cells and/or stabilise encysted cells. The present disclosure also relates to methods of infecting and/or colonising molluscs with the compositions and ciliates described herein.

公开(公告)号：US11920153B2

公开(公告)日：2024-03-05

申请号：US16339164

申请日：2017-10-03

申请人： Alma Mater Studiorum—Università di Bologna

发明人： Stefania Rapino ,  Francesco Zerbetto ,  Gastone Castellani ,  Stefano Salvioli ,  Beatrice Fraboni ,  Isabella Zironi ,  Maria Conte ,  Claudio Franceschi ,  Daniela Salvatore ,  Maila Becconi

IPC分类号： C12N5/00 ,  C12N5/09 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/08 ,  C12N11/04 ,  C12N11/06 ,  C12N11/087 ,  C12N11/089 ,  C12N11/12 ,  G01N33/50

CPC分类号： C12N5/0018 ,  C12N5/0068 ,  C12N5/0693 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0036 ,  C12N9/0053 ,  C12N9/0061 ,  C12N9/0065 ,  C12N9/0069 ,  C12N9/0093 ,  C12N11/04 ,  C12N11/06 ,  C12N11/087 ,  C12N11/089 ,  C12N11/12 ,  C12Y101/03004 ,  C12Y106/03001 ,  C12Y109/03001 ,  C12Y110/03002 ,  C12Y111/01006 ,  C12Y113/12004 ,  C12Y117/03002 ,  G01N33/5008 ,  C12N2500/02 ,  C12N2501/71 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2533/72 ,  C12N2533/74 ,  C12N2533/76 ,  C12N2533/78 ,  C12N2533/80 ,  C12N2537/10

摘要： The present invention relates to compositions comprising a polymeric matrix or a gel containing functional enzymes capable of re-creating under culture conditions the cell microenvironment existing in vivo. The present invention also relates to devices for cell cultures comprising such compositions, in particular hydrogel and the use thereof to control the chemical microenvironment of a cell culture or mimic physiological or pathological conditions of the in vivo cells. The compositions and the devices described herein could be also used in vitro for evaluating the therapeutic effect of a compound on a determined cell line or on primary cells.

公开(公告)号：US11892410B2

公开(公告)日：2024-02-06

申请号：US17051631

申请日：2019-02-20

申请人： Kyungpook National University Industry-Academic Cooperation Foundation

发明人： Soo Young Park ,  Kyung Gyu Noh

IPC分类号： G01N21/75 ,  C09K19/58 ,  C12N11/04 ,  C12Q1/58 ,  C12N9/80 ,  C08J3/075 ,  G01N21/80

CPC分类号： G01N21/75 ,  C08J3/075 ,  C09K19/586 ,  C12N9/80 ,  C12N11/04 ,  C12Q1/58 ,  G01N21/80 ,  C08J2333/08

摘要： The present invention relates to a method for preparing a solid-state photonic crystal IPN composite functionalized with an enzyme, a photonic crystal IPN composite prepared by the method, and a biosensor using the photonic crystal IPN composite. The method of the present invention includes (1) mixing a nonreactive chiral dopant with a reactive nematic mesogen, curing the mixture, and removing the chiral dopant while maintaining a helical structure, to form a solid-state helical photonic crystal structure, (2) infiltrating a PAA hydrogel into the internal space of the photonic crystal structure, followed by curing to form an IPN-structured composite, and (3) immobilizing an enzyme in the IPN-structured composite. The PAA hydrogel is infiltrated into the solid-state helical photonic crystal structure and the enzyme is immobilized such that a pH change caused by the enzymatic reaction induces shrinkage and expansion of the PAA hydrogel, leading to a color change.

公开(公告)号：US11719606B2

公开(公告)日：2023-08-08

申请号：US16628595

申请日：2018-07-03

申请人： University Public Corporation Osaka

发明人： Chie Kojima ,  Akikazu Matsumoto

IPC分类号： G01N1/30 ,  C12N11/04 ,  C12N11/08 ,  G01N33/48

CPC分类号： G01N1/30 ,  C12N11/04 ,  C12N11/08 ,  G01N33/48

摘要： The present invention provides a method for rapidly clearing a biological tissue. According to the present invention, provided is a method for clearing a biological tissue, including the steps of: infiltrating the biological tissue with water-soluble ethylenically unsaturated monomers before, during or after fixing the biological tissue with a fixative, wherein the water-soluble ethylenically unsaturated monomers include at least a water-soluble ethylenically unsaturated monomer having an ionically dissociable group; polymerizing the water-soluble ethylenically unsaturated monomers to form a hydrogel in the fixed biological tissue; and removing a lipid from the fixed biological tissue.

公开(公告)号：US20230193514A1

公开(公告)日：2023-06-22

申请号：US18150519

申请日：2023-01-05

申请人： Northeast Agricultural University

发明人： Juncai HOU ,  Jiage MA ,  Zhanmei JIANG ,  Cong XU ,  Wan WANG

IPC分类号： D01D5/00 ,  A61K9/00 ,  C12N1/20 ,  C12N11/04 ,  D01F1/10

CPC分类号： D01D5/0007 ,  A61K9/0092 ,  C12N1/20 ,  C12N11/04 ,  D01F1/10 ,  D01D5/0076

摘要： A probiotic-encapsulating gum Arabic (GA) composite fiber/capsule, including: lactic acid bacteria and an electrospun fiber or electrosprayed capsule, where the lactic acid bacteria are encapsulated in the electrospun fiber or electrosprayed capsule by electro-hydro dynamics (EHD) technology. The electrospun fiber or electrosprayed capsule is prepared by compounding a polymer matrix with GA, and the polymer matrix is polyvinyl alcohol (PVOH), polyvinylpyrrolidone (PVP), whey protein concentrate (WPC) or maltodextrin (MD). A preparation method and an application of the composite fiber/capsule are also provided.

公开(公告)号：US20230193236A1

公开(公告)日：2023-06-22

申请号：US18110715

申请日：2023-02-16

申请人： EVOCO LTD.

发明人： JASON JAMES ROBINSON ,  NATALIE BETH ASHDOWN ,  GREGORY ALLAN WHITTON

IPC分类号： C12N11/093 ,  C08G18/42 ,  C08G18/76 ,  C12N11/04

CPC分类号： C12N11/093 ,  C08G18/4238 ,  C08G18/7671 ,  C12N11/04 ,  C08G2110/0066 ,  C08G2110/0008 ,  C08G2110/005 ,  C08G2110/0058 ,  C08G2101/00

摘要： A biodegradable foam which includes a polyester-based polyurethane foam and a mixture comprised of a soil-dwelling carbon-digesting bacteria embedded in a carrier compound. The mixture of the soil-dwelling carbon-digesting bacteria is homogenously dispersed throughout the polyester-based polyurethane foam. This biodegradable foam exhibits biodegradation rates higher than a polyester-based polyurethane foam absent the soil-dwelling carbon-digesting bacteria.

公开(公告)号：US20230193181A1

公开(公告)日：2023-06-22

申请号：US17999286

申请日：2021-05-20

申请人： Oxford University Innovation Limited

发明人： John Hagan Pryce Bayley ,  Linna Zhou

IPC分类号： C12M1/12 ,  C12M1/26 ,  C12N11/04

CPC分类号： C12M25/01 ,  C12M25/16 ,  C12M33/00 ,  C12N11/04

摘要： The invention relates to an apparatus for producing a droplet assembly, which apparatus comprises: at least one droplet generator suitable for generating droplets of a viscous droplet medium; a droplet receiving region which is moveable relative to the at least one droplet generator; a temperature controller; and a control unit, which control unit is adapted to control the dispensing of droplets from the at least one droplet generator and the movement of the droplet receiving region relative to the at least one droplet generator, wherein the apparatus is adapted to produce a droplet assembly in the droplet receiving region, wherein the droplet assembly comprises a plurality of droplets, wherein each of said droplets comprises (i) a droplet medium, and (ii) an outer layer of amphipathic molecules around the surface of the droplet medium.

公开(公告)号：US10072257B2

公开(公告)日：2018-09-11

申请号：US14381866

申请日：2013-02-28

申请人： Massachusetts Institute of Technology

发明人： Sangeeta N. Bhatia ,  Kelly R. Stevens

IPC分类号： C12N5/00 ,  C12N11/04 ,  C12N5/071 ,  A61K35/12 ,  C12N11/08 ,  C12N11/10 ,  G01N33/50

CPC分类号： C12N11/04 ,  A61K35/12 ,  C12N5/0012 ,  C12N5/0062 ,  C12N5/0671 ,  C12N11/08 ,  C12N11/10 ,  C12N2533/76 ,  C12N2535/10 ,  G01N33/5005

摘要： The invention features an “inverse patterning” or “Intaglio-Void/Embed-Relief Topographic (In VERT) molding” manufacturing process for generating high-resolution three-dimensional (3D) multi-cellular microstructures in distinct cellular compartments of a single hydrogel. The platform has general utility in the development of engineered tissues for human therapies, drug testing, and disease models. Additionally, the platform can serve as a model system for studying 3D cell-cell interactions in fields as diverse as stem cell biology to the development of cancer therapeutics.