公开(公告)号：US20200325188A1

公开(公告)日：2020-10-15

申请号：US16622301

申请日：2018-06-13

Applicant: Aalto University Foundation sr

Inventor： Markus Linder ,  Pezhman Mohammadi ,  Sesilja Aranko

IPC: C07K14/435 ,  C07K1/02 ,  C07K14/33 ,  D01D1/02 ,  D01F4/00

Abstract: The present invention is directed to a method for producing a condensed phase of a silk fusion protein, the method comprising the steps of preparing a solution of a silk fusion protein in an aqueous medium and concentrating said silk fusion protein in said aqueous medium, wherein said fusion protein is isolated from a recombinant production host and comprises a silk-like protein sequence and two separate non-silk terminal module sequences, such as cellulose binding modules, SpyCatcher domains, tenth type III module of Fibronectin, gamma-crystallin D, flanking said silk-like protein sequence; wherein the method is performed so that said silk fusion protein is not precipitated and subsequently dissolved to said aqueous medium. The present invention is also directed to using such fusion proteins as adhesives.

公开(公告)号：US11597750B2

公开(公告)日：2023-03-07

申请号：US16622301

申请日：2018-06-13

Applicant: Aalto University Foundation sr

Inventor： Markus Linder ,  Pezhman Mohammadi ,  Sesilja Aranko

IPC: C07K14/435 ,  C07K1/02 ,  C07K14/33 ,  D01D1/02 ,  D01F4/00 ,  C12N15/62 ,  A61L27/36

Abstract: The present invention is directed to a method for producing a condensed phase of a silk fusion protein, the method comprising the steps of preparing a solution of a silk fusion protein in an aqueous medium and concentrating the silk fusion protein in the aqueous medium, wherein the fusion protein is isolated from a recombinant production host and comprises a silk-like protein sequence and two separate non-silk terminal module sequences, such as cellulose binding modules, SpyCatcher domains, tenth type III module of Fibronectin, gamma-crystallin D, flanking the silk-like protein sequence; wherein the method is performed so that the silk fusion protein is not precipitated and subsequently dissolved to the aqueous medium. The present invention is also directed to using such fusion proteins as adhesives.