公开(公告)号：US12221613B2

公开(公告)日：2025-02-11

申请号：US17923455

申请日：2021-04-29

Applicant: Academia Sinica

Inventor： Su-Ying Yeh ,  Hsin-Hung Lin ,  Maurice S. B. Ku ,  Wen-Hsiung Li

IPC: C12N15/82

Abstract: A transgenic rice plant containing in its genome a recombinant DNA construct that includes a first nucleic acid having a sequence of a first Golden 2-like transcription factor (GLK) gene operably linked to its natural promoter and 5′ untranslated region (5′UTR), and a second nucleic acid having a sequence of a second GLK gene operably linked to its natural promoter and 5′UTR, the second GLK gene being distinct from the first GLK gene. The first GLK gene and the second GLK gene are both from a C4 plant and the transgenic rice plant exhibits a 65-106% increase in shoot biomass and a 50-95% increase in grain yield, as compared to an untransformed wild-type rice plant. Also provided is a method for producing the transgenic rice plant and a recombinant DNA construct that can be used in the method.

公开(公告)号：US20230203521A1

公开(公告)日：2023-06-29

申请号：US17923455

申请日：2021-04-29

Applicant: Academia Sinica

Inventor： Su-Ying Yeh ,  Hsin-Hung Lin ,  Maurice S. B. Ku ,  Wen-Hsiung Li

IPC: C12N15/82

CPC classification number: C12N15/8269 ,  C12N15/8265

Abstract: A transgenic rice plant containing in its genome a recombinant DNA construct that includes a first nucleic acid having a sequence of a first Golden 2-like transcription factor (GLK) gene operably linked to its natural promoter and 5′ untranslated region (5′UTR), and a second nucleic acid having a sequence of a second GLK gene operably linked to its natural promoter and 5′UTR, the second GLK gene being distinct from the first GLK gene. The first GLK gene and the second GLK gene are both from a C4 plant and the transgenic rice plant exhibits a 65-106% increase in shoot biomass and a 50-95% increase in grain yield, as compared to an untransformed wild-type rice plant. Also provided is a method for producing the transgenic rice plant and a recombinant DNA construct that can be used in the method.