公开(公告)号：US20180030473A1

公开(公告)日：2018-02-01

申请号：US15723312

申请日：2017-10-03

Applicant: Academia Sinica

Inventor： SWEE-SUAK KO ,  MIN-JENG LI

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8289 ,  C07K14/415

Abstract: The present invention relates to a method for producing male sterile plant, a mutated nucleotide molecule comprising a nucleotide sequence of the transcription factor bHLH142 and an inserted T-DNA segment, and a novel transformed plant cell and a male-sterile mutant plant comprising the mutated nucleotide molecule, in which the transcription factor bHLH142 is not expressed. The present invention also relates to a novel reversible male sterile transgenic plant, wherein the transcription factor bHLH142 is overexpressed, and its preparation method. The bHLH gene is tissue specifically expresses in the anther and it plays a pivotal role in pollen development. Both the male sterile and reversible male sterile transgenic plants showed a completely male sterile phenotype, but the fertility of the reversible male sterile transgenic plant can be restored under low temperature.

公开(公告)号：US20150315607A1

公开(公告)日：2015-11-05

申请号：US14596419

申请日：2015-01-14

Applicant: Academia Sinica

Inventor： SWEE-SUAK KO ,  MIN-JENG LI

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8289 ,  C07K14/415

Abstract: The present invention relates to a method for producing male sterile plant, a mutated nucleotide molecule comprising a nucleotide sequence of the transcription factor bHLH142 and an inserted T-DNA segment, and a novel transformed plant cell and a male-sterile mutant plant comprising the mutated nucleotide molecule, in which the transcription factor bHLH142 is not expressed. The present invention also relates to a novel reversible male sterile transgenic plant, wherein the transcription factor bHLH142 is overexpressed, and its preparation method. The bHLH gene is tissue specifically expresses in the anther and it plays a pivotal role in pollen development. Both the male sterile and reversible male sterile transgenic plants showed a completely male sterile phenotype, but the fertility of the reversible male sterile transgenic plant can be restored under low temperature.

Abstract translation: 本发明涉及一种生产雄性不育植物的方法，包含转录因子bHLH142的核苷酸序列和插入的T-DNA片段的突变核苷酸分子，以及新的转化植物细胞和雄性不育突变植物，其包含突变的 核苷酸分子，其中转录因子bHLH142未被表达。 本发明还涉及一种新型的可逆雄性不育转基因植物，其中转录因子bHLH142被过表达，其制备方法。 bHLH基因在花药中特异性表达，在花粉发育中起关键作用。 雄性不育和可逆雄性不育转基因植物均显示完全雄性不育表型，但可逆性雄性不育转基因植物的生育力可在低温下恢复。