公开(公告)号：US20250155401A1

公开(公告)日：2025-05-15

申请号：US18916683

申请日：2024-10-15

Applicant: Advanced Biomedical Instrumentation Centre Limited

Inventor： William Lawrence WHITEHOUSE ,  Hoi Ying Louisa LO ,  Julian Alexander TANNER

IPC: G01N27/327 ,  G01N33/543

Abstract: An electrochemical biosensing method using screen-printed gold electrodes functionalized with a redox probe modified DNA aptamer that binds specifically to CRP is provided. Binding-induced conformational switching of the CRP-targeting aptamer induces a specific and selective signal-ON event, which enables single-step and reagentless detection of CRP in as little as 1 minute. The aptasensor limit of detection spans approximately 20-60 nM in 50% human serum with dynamic response windows spanning 1-200 or 1-500 nM (R=0.97/R=0.98 respectively). The sensor also operates in undiluted human blood producing an LOD of 45 nM equating to 5.4 mg/L. The sensor is stable for at least 1 week and can be reused numerous times, as judged from repeated real-time dosing and dose-response assays.