公开(公告)号：US20160010127A1

公开(公告)日：2016-01-14

申请号：US14379075

申请日：2013-02-18

Applicant: Agency for Science, Technology and Research

Inventor： Jinchuan WU ,  Zhibin LIU ,  Han Ling CHOW

IPC: C12P19/14 ,  C12P7/56 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P7/56 ,  C12P19/02 ,  C12P2203/00

Abstract: The present invention relates to methods for enzymatic hydrolysis of cellulose using whole cell cultures. The cellulase-producing microbes (e.g. fungus) may be cultivated at a lower temperature (e.g. about 30° C.) to produce extracellular cellulase enzymes followed by raising the temperature to a higher level (e.g. about 50° C.) to deactivate the cells and to promote the cellulose hydrolysis by extracellular cellulases resulting in continuous hydrolysis of cellulose to glucose without the risk of glucose being consumed by the deactivated cells.

Abstract translation: 本发明涉及使用全细胞培养物酶促水解纤维素的方法。 产生纤维素酶的微生物（例如真菌）可以在较低温度（例如约30℃）下培养以产生细胞外纤维素酶，随后将温度升高到更高的水平（例如约50℃）以使细胞失活 并通过胞外纤维素酶促进纤维素水解，导致纤维素连续水解成葡萄糖，而不会被失活的细胞消耗葡萄糖的风险。