公开(公告)号：US20150219643A1

公开(公告)日：2015-08-06

申请号：US14613649

申请日：2015-02-04

Applicant: Agency for Science, Technology and Research

Inventor： Hong Yan Song ,  Ten It Wong ,  Anton Valentinovich Sadovoy ,  Xiaodong Zhou ,  Ping Bai ,  Lin Wu

IPC: G01N33/543 ,  G01N21/65 ,  G01N33/58 ,  G01N33/553

CPC classification number: G01N33/582 ,  G01N21/648 ,  G01N33/553 ,  G01N33/588

Abstract: There is method for detecting the presence or absence of a target analyte in a sample comprising the steps of: allowing an inorganic fluorescent particle to conjugate with a detection biomolecule that is bound to said target analyte, if present in said sample, said target analyte additionally being bound by a capture biomolecule immobilized on or within a nanostructure; and detecting the fluorescence emitted by said inorganic fluorescent particle, said nanostructure being capable of generating a surface plasmon resonance effect when excited by a light source to substantially increase the fluorescence emitted by said inorganic fluorescent particle, wherein the detected fluorescence indicates the presence of said target analyte in said sample.

Abstract translation: 存在用于检测样品中目标分析物的存在或不存在的方法，包括以下步骤：使无机荧光颗粒与结合到所述目标分析物的检测生物分子（如果存在于所述样品中）共轭，则所述靶分析物另外 被固定在纳米结构上或内部的捕获生物分子结合; 并且检测由所述无机荧光颗粒发射的荧光，所述纳米结构当被光源激发时能够产生表面等离子体共振效应，以显着增加由所述无机荧光颗粒发射的荧光，其中所检测的荧光指示存在所述目标 所述样品中的分析物。

公开(公告)号：US09995749B2

公开(公告)日：2018-06-12

申请号：US14613649

申请日：2015-02-04

Applicant: Agency for Science, Technology and Research

Inventor： Hong Yan Song ,  Ten It Wong ,  Anton Valentinovich Sadovoy ,  Xiaodong Zhou ,  Ping Bai ,  Lin Wu

IPC: G01N33/58 ,  G01N33/553 ,  G01N21/64

CPC classification number: G01N33/582 ,  G01N21/648 ,  G01N33/553 ,  G01N33/588

Abstract: There is method for detecting the presence or absence of a target analyte in a sample comprising the steps of: allowing an inorganic fluorescent particle to conjugate with a detection biomolecule that is bound to said target analyte, if present in said sample, said target analyte additionally being bound by a capture biomolecule immobilized on or within a nanostructure; and detecting the fluorescence emitted by said inorganic fluorescent particle, said nanostructure being capable of generating a surface plasmon resonance effect when excited by a light source to substantially increase the fluorescence emitted by said inorganic fluorescent particle, wherein the detected fluorescence indicates the presence of said target analyte in said sample.