公开(公告)号：US20150192589A1

公开(公告)日：2015-07-09

申请号：US14592381

申请日：2015-01-08

Applicant: Agency for Science, Technology and Research

Inventor： Meng Gao ,  Bin Liu ,  Kai Li

IPC: G01N33/58 ,  C12Q1/34 ,  G01N33/50 ,  C12Q1/44

CPC classification number: G01N33/582 ,  C12Q1/34 ,  C12Q1/44 ,  G01N33/5079 ,  G01N2333/92 ,  G01N2333/938

Abstract: The invention provides method for imaging the activity of enzymes or bioactive molecules in cells using fluorescent probes with aggregation-induced emission properties and excited-state intramolecular proton transfer properties. The fluorescent probes prepared according to the invention are those of formula (I), wherein W, X, Y, Z have the meaning as described in the description, R is a reactive group that can interact with enzymes and other bioactive molecules, Linker is a single bond or a combination of chemical bonds linking the targeting group T to the probe molecule, and T represents a targeting group that has an ability to interact with an organelle.

Abstract translation: 本发明提供了使用具有聚集诱导的发射特性和激发态分子内质子转移特性的荧光探针对细胞中酶或生物活性分子的活性进行成像的方法。 根据本发明制备的荧光探针是式（I）的荧光探针，其中W，X，Y，Z具有描述中所述的含义，R是可与酶和其它生物活性分子相互作用的反应性基团，连接子是 单一键或将靶向基团T连接到探针分子的化学键的组合，T表示具有与细胞器相互作用的能力的靶向基团。