公开(公告)号：US6071728A

公开(公告)日：2000-06-06

申请号：US331899

申请日：1999-10-18

申请人： Aharon M. Eyal ,  Vitner Asher ,  Pierre Cami ,  Robert Jansen ,  Bruno Jarry ,  Didier Lecomte ,  Jean Scott ,  Thomas Chattaway ,  Frank Van Lancker

发明人： Aharon M. Eyal ,  Vitner Asher ,  Pierre Cami ,  Robert Jansen ,  Bruno Jarry ,  Didier Lecomte ,  Jean Scott ,  Thomas Chattaway ,  Frank Van Lancker

IPC分类号： C08G69/10 ,  C08G73/10 ,  C12P7/46 ,  C12P13/20 ,  C07C227/00 ,  C07C229/00

CPC分类号： C12P13/20 ,  C08G69/10 ,  C08G73/1092 ,  C12P7/46

摘要： The invention provides a process for the preparation of aspartic acid via a fermentation process for the preparation of ammonia fumarate, wherein the pH of the fermentation broth is controlled by the addition of a calcium base to produce a calcium fumarate precipitate, characterized in that ammonium fumarate is produced by separating the precipitated calcium fumarate from the fermentation broth, and reacting the same with a reagent selected from ammonia, ammonium carbonate, ammonia in combination with CO.sub.2 and mixtures therefore, to form ammonium fumarate and a co-product selected from calcium carbonate and calcium hydroxide, wherein the energy of indirect neutralization of fumaric acid by ammonia serves as the driving force for the conversion of calcium fumarate to the desired ammonium fumarate product and for the regeneration of a calcium base reagent, and wherein diammonium fumarate is enzymatically converted to ammonium aspartate and acidulated to from aspartic acid.

摘要翻译： PCT No.PCT / US98 / 00290 Sec。 371 1999年10月18日第 102（e）1999年10月18日PCT PCT 1998年1月8日PCT公布。 出版物WO98 / 30712 PCT 日期1998年7月16日本发明提供了一种通过用于制备富马酸铵的发酵方法制备天冬氨酸的方法，其中通过加入钙碱来控制发酵液的pH以产生富马酸钙沉淀物， 其特征在于富马酸铵是通过从发酵液中分离出沉淀的富马酸钙，并将其与选自氨，碳酸铵，氨与二氧化碳和其混合物的试剂反应而生成的，从而形成富马酸铵和共产物 选自碳酸钙和氢氧化钙，其中通过氨间接中和富马酸的能量用作将富马酸钙转化为所需的富马酸铵产物和再生钙碱试剂的驱动力，并且其中富马酸二铵 酶促转化为天冬氨酸铵，并酸化至天冬氨酸 d。

公开(公告)号：US06344348B1

公开(公告)日：2002-02-05

申请号：US09284234

申请日：1999-11-22

申请人： Aharon Eyal ,  Robert J. Jansen ,  Asher Vitner ,  Pierre Cami ,  Emmanuel Mailly ,  Thomas Chattaway ,  Bruno Jarry ,  Joelle More

发明人： Aharon Eyal ,  Robert J. Jansen ,  Asher Vitner ,  Pierre Cami ,  Emmanuel Mailly ,  Thomas Chattaway ,  Bruno Jarry ,  Joelle More

IPC分类号： C12P1320

CPC分类号： C12P13/20 ,  C08G73/1092

摘要： The invention provides a process for the production of aspartic acid poly-condensate from a carbohydrate comprising the steps of: fermenting a carbohydrate-containing medium by means of a fumaric acid-producing microorganism, whereby a fumarate-containing fermentation liquor is formed; forming a purified ammonium fumarate solution from the fumarate-containing fermentation liquor; enzymatically converting the purified ammonium fumarate into purified ammonium aspartate; heating an aqueous solution of an aspartate salt derived from the purified ammonium aspartate whereby water is removed, an aspartic acid condensate is formed and a second product is formed, which second product is basic and contains the cation of the aspartate salt; and removing and using the basic second product as a reagent in another step of the process.

摘要翻译： 本发明提供了一种从碳水化合物制备天冬氨酸聚缩合物的方法，包括以下步骤：通过产生富马酸的微生物发酵含碳水化合物的培养基，从而形成含富马酸的发酵液; 从含富马酸的发酵液中形成纯化的富马酸铵溶液; 将纯化的富马酸铵酶促转化成纯天然天冬氨酸铵; 加热源自纯化天冬氨酸铵的天冬氨酸盐的水溶液，由此除去水，形成天冬氨酸缩合物并形成第二产物，该第二产物是碱性的，并含有天冬氨酸盐的阳离子; 以及在该方法的另一步骤中除去和使用碱性第二产物作为试剂。