公开(公告)号：US20240360486A1

公开(公告)日：2024-10-31

申请号：US18649779

申请日：2024-04-29

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Deepika AWASTHI ,  Shubhasish GOSWAMI ,  Steven W. SINGER

IPC分类号： C12P7/46 ,  C12N1/20 ,  C12N9/16 ,  C12R1/01

CPC分类号： C12P7/46 ,  C12N1/205 ,  C12N9/16 ,  C12Y301/02018 ,  C12R2001/01

摘要： The present invention provides for a genetically modified host cell comprising one or more enzymes described herein, wherein the genetically modified host cell is capable of producing malonate from methane.

公开(公告)号：US20240309417A1

公开(公告)日：2024-09-19

申请号：US18413954

申请日：2024-01-16

申请人： Ramon Gonzalez

发明人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

IPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/00 ,  C12P13/04

CPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0077 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P5/02 ,  C12P5/026 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/001 ,  C12P13/04 ,  C12Y101/01 ,  C12Y101/03039 ,  C12Y102/01 ,  C12Y102/04001 ,  C12Y103/05001 ,  C12Y103/99 ,  C12Y108/01004 ,  C12Y114/15003 ,  C12Y203/01012 ,  C12Y203/01054 ,  C12Y207/01031 ,  C12Y208/03 ,  C12Y401/00 ,  C12Y401/01 ,  C12Y401/01047 ,  C12Y401/03024 ,  C12Y402/01 ,  C12Y402/01002 ,  C12Y402/01011 ,  C12Y501/99001 ,  C12Y501/99002 ,  C12Y504/02 ,  C12Y504/99 ,  C12Y504/99001 ,  C12Y504/99002 ,  C12Y602/01 ,  C12Y602/01005 ,  C12Y604/01003 ,  Y02E50/30

摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

公开(公告)号：US20240240210A9

公开(公告)日：2024-07-18

申请号：US18188995

申请日：2023-03-23

申请人： China Petrochemical Development Corporation, Taipei (Taiwan) ,  NATIONAL CHEN KUNG UNIVERSITY

发明人： I-Son NG ,  Jo-Shu CHANG ,  Chuan-Chieh HSIANG ,  Yeong-Chang CHEN ,  Yu-Chiao LIU ,  Chia-Wei TSAI

IPC分类号： C12P7/46 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70

CPC分类号： C12P7/46 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12R2001/19 ,  C12Y401/01006 ,  C12Y402/01003

摘要： Provided is a recombinant microorganism including at least two genes for producing itaconic acid and its derived monomers, and the at least two genes are located on the same expression vector. The at least two genes include one encoding cis-aconitic acid decarboxylase and the other one encoding aconitase, and the genome of the recombinant microorganism includes a gene encoding the molecular chaperone protein GroELS. Also provided is a method for producing itaconic acid by using the microorganism.

公开(公告)号：US20230272440A1

公开(公告)日：2023-08-31

申请号：US18006316

申请日：2020-12-21

申请人： Cathy Staloch HASS ,  Peter Alan JAUERT ,  Nandita KOHLI ,  Eric Michael LENNEMAN ,  Hans H. LIAO ,  Catherine Bradshaw POOR ,  Brian Jeffrey RUSH ,  Robbyn Lynn WEAVER ,  CARGILL, INCORPORATED

发明人： Cathy Staloch HASS ,  Peter Alan JAUERT ,  Nandita KOHLI ,  Panagiota KYRIAKOU ,  Eric Michael LENNEMAN ,  Hans H. LIAO ,  Catherine Bradshaw POOR ,  Brian Jeffrey RUSH ,  Robbyn Lynn WEAVER

IPC分类号： C12P7/46 ,  C12N9/02

CPC分类号： C12P7/46 ,  C12N9/0008 ,  C12Y102/01027

摘要： The present disclosure provides an engineered microorganism capable of producing malonic acid, malonate, esters of malonic acid, or mixtures thereof. The engineered microorganism includes a malonate-semialdehyde dehydrogenase that is heterologous to a native form of the engineered microorganism and comprises at least 80% sequence identity to SEQ ID NO: 6, wherein the engineered microorganism is capable of producing about 3 g/L to about 250 g/L of malonic acid, malonate, esters of malonic acid, or mixtures thereof.

公开(公告)号：US11692187B2

公开(公告)日：2023-07-04

申请号：US16472514

申请日：2017-12-21

申请人： VIB vzw ,  Katholieke Universiteit Leuven, K.U. Leuven R&D ,  GlobalYeast N.V.

发明人： Johan Thevelein ,  Mekonnen Demeke ,  Maria Remedios Foulquié Moreno ,  Stijn De Graeve ,  Edgard Valdomiro Charles Belo

IPC分类号： C12N9/90 ,  C12N15/87 ,  C12N9/92 ,  C12P5/02 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/6409 ,  C12P13/04 ,  C12P35/00 ,  C12N15/90

CPC分类号： C12N9/92 ,  C12N15/87 ,  C12N15/905 ,  C12P5/026 ,  C12P7/10 ,  C12P7/16 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/6409 ,  C12P13/04 ,  C12P35/00 ,  C12Y503/01005

摘要： The present invention relates to novel nucleic acid sequences encoding bacterial xylose isomerases that upon transformation of a eukaryotic microbial host cell, such as yeast, to confer to the host cell the ability of isomerising xylose to xylulose. The nucleic acid sequences encode xylose isomerases that originate from bacteria such as Eubacterium sp., Clostridium cellulosi and others. The invention further relates to fermentation processes wherein the transformed host cells ferment a xylose-containing medium to produce ethanol or other fermentation products.

公开(公告)号：US20230193299A1

公开(公告)日：2023-06-22

申请号：US17812875

申请日：2022-07-15

申请人： CARGILL, INCORPORATED

发明人： Kenneth R. FINLEY ,  Jeanette M. HURYTA ,  Beth M. MASTEL ,  Thomas W. McMULLIN ,  Gregory M POYNTER ,  Brian J. RUSH ,  Arlene M. FOSMER ,  Vernon L. McINTOSH, Jr. ,  Keith M. BRADY ,  Kevin T. WATTS

IPC分类号： C12N15/81 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/00 ,  C12P7/46 ,  C12N15/52

CPC分类号： C12N15/815 ,  C12N9/0006 ,  C12N9/001 ,  C12Y604/01001 ,  C12Y101/01037 ,  C12Y103/01006 ,  C12N9/88 ,  C12N9/93 ,  C12Y402/01002 ,  C12P7/46 ,  C12N15/52 ,  Y02P20/52

摘要： The present application provides genetically modified yeast cell comprising an active succinate fermentation pathway, as well as methods of using these cells to produce succinate.

公开(公告)号：US20190093135A1

公开(公告)日：2019-03-28

申请号：US16086883

申请日：2017-03-21

申请人： UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.

发明人： Xianghao Wu ,  Mark Eiteman

IPC分类号： C12P7/46 ,  C12N9/10

CPC分类号： C12P7/46 ,  C12N9/10 ,  C12N9/1025 ,  C12N9/1029 ,  C12N15/70 ,  C12Y203/01182 ,  C12Y203/03001

摘要： Provided herein is a genetically engineered microbe which accumulates citramalate. In one embodiment, the microbe includes an exogenous polynucleotide encoding a citramalate synthase which catalyzes the condensation of acetyl CoA and pyruvic acid. Optionally, the microbe also includes a second exogenous polynucleotide encoding a citrate synthase which catalyzes the condensation of acetyl CoA and oxaloacetate, and the citrate synthase activity in the microbe is reduced compared to a control microbe. In one embodiment, the citrate synthase includes at least one amino acid substitution in the acetyl-CoA binding pocket, the mobile loop, the NADH binding site, and the oxaloacetate binding site, or a combination thereof. Also provided herein are methods for using the genetically engineered microbe, including a method for producing citramalate. The method can further include isolating the citramalate.

公开(公告)号：US20180305656A1

公开(公告)日：2018-10-25

申请号：US15495625

申请日：2017-04-24

申请人： BATTELLE ENERGY ALLIANCE, LLC

发明人： LYNN M. WENDT ,  BRADLEY D. WAHLEN ,  CHENLIN LI

IPC分类号： C12N1/12 ,  C12P7/46 ,  C12N1/20

CPC分类号： C12N1/12 ,  C12N1/20 ,  C12P7/46

摘要： A method of preserving a biomass. The method comprises adding an acid solution to a biomass comprising microalgae to form an acidified microalgae biomass composition. The acidified microalgae biomass composition is stored under anaerobic conditions without inoculating the acidified microalgae biomass composition with bacteria. An additional method of preserving a biomass comprises storing the acidified microalgae biomass composition under anaerobic conditions and exposing the acidified microalgae biomass composition to carbon dioxide, nitrogen, or a combination thereof to produce a coproduct comprising succinic acid. Yet another method of preserving a biomass comprises storing the acidified microalgae biomass composition under anaerobic conditions and in the presence of carbon dioxide. A preserved biomass is also disclosed.

公开(公告)号：US10077437B2

公开(公告)日：2018-09-18

申请号：US15189021

申请日：2016-06-22

申请人： Yeda Research and Development Co. Ltd.

发明人： Ron Milo ,  Arren Bar-Even ,  Elad Noor

IPC分类号： C12N9/00 ,  C12N15/82 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12N9/10 ,  C12N9/18 ,  C12N9/88 ,  C12N9/90 ,  C12P7/40 ,  C12N15/70 ,  C12P7/42 ,  C12P7/46 ,  C12P11/00 ,  G06F19/12

CPC分类号： C12N9/93 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1018 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/18 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8269 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P11/00 ,  C12Y101/01059 ,  C12Y102/01018 ,  C12Y201/03001 ,  C12Y208/03001 ,  C12Y401/01031 ,  C12Y402/01017 ,  C12Y402/01054 ,  C12Y604/01001 ,  C12Y604/01002 ,  G16B5/00 ,  Y02P20/52

摘要： A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.

公开(公告)号：US20180245106A1

公开(公告)日：2018-08-30

申请号：US15314489

申请日：2015-05-28

申请人： DSM IP Assets B.V.

发明人： Zheng ZHAO

IPC分类号： C12P7/44 ,  C12P7/62

CPC分类号： C12P7/44 ,  C12P7/46 ,  C12P7/62

摘要： The present invention relates to a method for the production of itaconic acid or an ester of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid or an ester of itaconic acid in a suitable fermentation medium under anaerobic conditions, wherein the itaconic acid or ester of itaconic acid is produced.