公开(公告)号：US06201116B1

公开(公告)日：2001-03-13

申请号：US09277354

申请日：1999-03-26

申请人： Alan S. Verkman ,  Joachim Biwersi ,  Sujatha Jayaraman

发明人： Alan S. Verkman ,  Joachim Biwersi ,  Sujatha Jayaraman

IPC分类号： C07H506

CPC分类号： C07D471/14 ,  C07D475/06 ,  C07D475/14 ,  G01N33/582 ,  G01N33/6872 ,  G01N33/84

摘要： The invention provides methods and compositions for measuring ion concentration inside a cell by measuring fluorescence of a compound of the general formula I. In particular embodiments, the measured ion is halide, particularly iodide, the cell contains a functional anion transport protein or channel, the method measures a change in fluorescence as a function of a predetermined condition such as the presence of a predetermined amount of a candidate modulator of ion transport in the cell (e.g. for drug screening) or the expression by the cell of a transgene (e.g. to assess the efficacy of gene therapy).

摘要翻译： 本发明提供了通过测量通式I化合物的荧光来测量细胞内的离子浓度的方法和组合物。在具体实施方案中，测量的离子是卤化物，特别是碘化物，细胞含有功能性阴离子转运蛋白或通道， 方法测量作为预定条件的函数的荧光变化，例如在细胞中存在预定量的离子迁移的候选调节剂（例如用于药物筛选）或转基因细胞的表达（例如评估 基因治疗的功效）。