公开(公告)号：US20060116340A1

公开(公告)日：2006-06-01

申请号：US10808042

申请日：2004-03-24

申请人： Alfred Lewin ,  David Bloom ,  Gregory Schultz ,  Sonal Tuli ,  Jia Liu

发明人： Alfred Lewin ,  David Bloom ,  Gregory Schultz ,  Sonal Tuli ,  Jia Liu

IPC分类号： A61K48/00 ,  C12N5/06 ,  C07H21/02

CPC分类号： C07K14/005 ,  C12N2510/02 ,  C12N2710/16622

摘要： Hammerhead ribozymes that target components critical to HSV replication (ICP4, UL20, UL30, and UL54) were synthesized and shown to efficiently cleave target RNA encoding a portion of these components in in vitro assays. Several cloned cell lines stably expressing these ribozymes were established. HSV-mediated plaque formation was dramatically reduced in cells stably transfected with an anti-HSV ribozyme compared to non-transfected cells.

摘要翻译： 合成针对HSV复制（ICP4，UL20，UL30和UL54）关键组分的锤头核酶，并显示出在体外测定中有效切割编码这些成分的一部分的靶RNA。 建立了稳定表达这些核酶的几种克隆细胞系。 与未转染细胞相比，用抗HSV核酶稳定转染的细胞中HSV介导的斑块形成显着降低。