公开(公告)号：US06783732B2

公开(公告)日：2004-08-31

申请号：US10199743

申请日：2002-07-19

申请人： Alfred P Madden ,  Jon Hoshizaki ,  Jason H. Halsey ,  Stephen E. Moring ,  Mark F. Oldham ,  Kevin S. Bodner

发明人： Alfred P Madden ,  Jon Hoshizaki ,  Jason H. Halsey ,  Stephen E. Moring ,  Mark F. Oldham ,  Kevin S. Bodner

IPC分类号： G01N3500

CPC分类号： B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L2400/049 ,  G01N35/0099 ,  G01N35/028 ,  G01N35/1074 ,  G01N2035/00485 ,  G01N2035/102 ,  Y10T436/25

摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

摘要翻译： 本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。 在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器 板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。 本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件 大量死体积的样品成分。 此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。 此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。 本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。