公开(公告)号：US06770462B1

公开(公告)日：2004-08-03

申请号：US10009315

申请日：2001-11-05

申请人： Alison F. Chalker ,  David J. Holmes ,  Robert Dwayne Lunsford ,  James Yigong Ge

发明人： Alison F. Chalker ,  David J. Holmes ,  Robert Dwayne Lunsford ,  James Yigong Ge

IPC分类号： C12P1934

CPC分类号： C12N15/10 ,  C07K14/3156 ,  C12N15/102

摘要： A method for inserting a cassette into a nucleic acid molecule to produce a modified nucleic acid molecule, e.g., a nucleic acid-cassette fusion, is described. The method involves the use of polymerase chain reaction, thereby permitting precise targeting of the desired site in the molecule, but does not require ligation. Also described is the use of this method for high throughput screening the resulting modified nucleic acid molecules.

摘要翻译： 描述了将盒插入核酸分子以产生修饰的核酸分子例如核酸盒融合体的方法。 该方法涉及使用聚合酶链反应，从而允许分子中所需位点的精确靶向，但不需要连接。 还描述了使用该方法对所得修饰的核酸分子进行高通量筛选。

公开(公告)号：US06294652B1

公开(公告)日：2001-09-25

申请号：US09487370

申请日：2000-01-18

申请人： Sanjoy Biswas ,  James Yigong Ge ,  David John Holmes ,  Karen A Ingraham ,  John Throup ,  Nicola Gail Wallis ,  Magdalena Zalacain

发明人： Sanjoy Biswas ,  James Yigong Ge ,  David John Holmes ,  Karen A Ingraham ,  John Throup ,  Nicola Gail Wallis ,  Magdalena Zalacain

IPC分类号： C01K100

CPC分类号： C12N9/1223 ,  C07K14/3156 ,  Y10T436/105831

摘要： The invention provides Response regulator polypeptides and polynucleotides encoding Response regulator polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing Response regulator polypeptides to screen for antibacterial compounds.

公开(公告)号：US06225087B1

公开(公告)日：2001-05-01

申请号：US09139064

申请日：1998-08-25

申请人： Sanjoy Biswas ,  James Yigong Ge ,  David John Holmes ,  Karen A Ingraham ,  John Throup ,  Nicola Gail Wallis ,  Magdalena Zalacain

发明人： Sanjoy Biswas ,  James Yigong Ge ,  David John Holmes ,  Karen A Ingraham ,  John Throup ,  Nicola Gail Wallis ,  Magdalena Zalacain

IPC分类号： C12N1500

CPC分类号： C12N9/1223 ,  C07K14/3156 ,  Y10T436/105831

摘要： The invention provides Response regulator polypeptides and polynucleotides encoding Response regulator polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing Response regulator polypeptides to screen for antibacterial compounds.

摘要翻译： 本发明提供了响应调节剂多肽和编码响应调节多肽的多核苷酸以及通过重组技术产生此类多肽的方法。 还提供了利用响应调节剂多肽筛选抗菌化合物的方法。