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公开(公告)号：US20110015084A1
公开(公告)日：2011-01-20
申请号：US12739683
申请日：2008-10-23
申请人： Allen Christian , Michael W. Petersen , Xudong Ye , David Alan Somers , Jennifer Anne Rinehart , LaRee Witte Frank , Shengzhi Pang , Amy Marie Nichols , John Alan Korte , Heping Yang
发明人： Allen Christian , Michael W. Petersen , Xudong Ye , David Alan Somers , Jennifer Anne Rinehart , LaRee Witte Frank , Shengzhi Pang , Amy Marie Nichols , John Alan Korte , Heping Yang
CPC分类号： C12Q1/6895 , C12Q1/6851 , Y10T436/143333 , C12Q2525/161 , C12Q2563/131
摘要： The present invention provides a high-throughput system for determining linkage of distinct polynucleotides and determining the sequence of polynucleotides that are linked to the distinct polynucleotides. The methods are particularly useful for analyzing transgenes in a transformed host organism. The disclosed methods provide for the detection of linkage between distinct transgenic polynucleotides in transformed hosts and sequencing of DNA regions linked to the distinct transgenic polynucleotides. Methods for identifying a transgenic plant containing a transgene insertion in an undesirable genomic location are also disclosed.
摘要翻译： 本发明提供了一种用于确定不同多核苷酸连接并确定与不同多核苷酸连接的多核苷酸序列的高通量系统。 所述方法对于分析转化宿主生物体中的转基因特别有用。 所公开的方法提供检测转化宿主中不同转基因多核苷酸之间的连接以及与不同转基因多核苷酸连接的DNA区域的测序。 还公开了用于鉴定在不期望的基因组位置中含有转基因插入的转基因植物的方法。
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2.发明申请Cotton event PV-GHBK04 (531) and compositions and methods for detection thereof 有权棉花事件PV-GHBK04（531）及其检测方法公开(公告)号：US20080124724A1
公开(公告)日：2008-05-29
申请号：US11801114
申请日：2007-05-08
CPC分类号： A01H5/10 , C12N15/8286 , Y02A40/162 , Y10S435/975
摘要： The present invention provides assays for detecting the presence of the 531 cotton event nucleic acid sequences in a biological sample based on the DNA sequence of the recombinant construct inserted into the cotton genome and of genomic sequences flanking the insertion site in a cotton genome.
摘要翻译： 本发明提供了基于插入到棉花基因组中的重组构建体的DNA序列和棉花基因组中插入位点侧翼的基因组序列来检测生物样品中531个棉花事件核酸序列的存在的测定。
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3.发明授权Cotton event PV-GHBK04 (531) and compositions and methods for detection thereof 有权棉花事件PV-GHBK04（531）及其检测方法公开(公告)号：US07964348B2
公开(公告)日：2011-06-21
申请号：US11801114
申请日：2007-05-08
CPC分类号： A01H5/10 , C12N15/8286 , Y02A40/162 , Y10S435/975
摘要： The present invention provides assays for detecting the presence of the 531 cotton event nucleic acid sequences in a biological sample based on the DNA sequence of the recombinant construct inserted into the cotton genome and of genomic sequences flanking the insertion site in a cotton genome.
摘要翻译： 本发明提供了基于插入到棉花基因组中的重组构建体的DNA序列和棉花基因组中插入位点侧翼的基因组序列来检测生物样品中531个棉花事件核酸序列的存在的测定。
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公开(公告)号：US5514570A
公开(公告)日：1996-05-07
申请号：US363560
申请日：1994-12-21
申请人： Dennis Gonsalves , Shengzhi Pang , John Hu
发明人： Dennis Gonsalves , Shengzhi Pang , John Hu
CPC分类号： C07K14/005 , C12N15/8283 , C12N2770/34022
摘要： Squash mosaic virus coat protein genes are disclosed. Expression vectors containing squash mosaic virus coat protein genes are disclosed. Transgenic plants that contain squash mosaic virus coat protein genes and that are resistant to infection by squash mosaic virus are disclosed.
摘要翻译： 披露了南瓜花叶病毒外壳蛋白基因。 公开了含有南瓜花叶病毒外壳蛋白基因的表达载体。 公开了含有南瓜花叶病毒外壳蛋白基因并且耐受南瓜花叶病毒感染的转基因植物。
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