公开(公告)号：US12013392B2

公开(公告)日：2024-06-18

申请号：US17464850

申请日：2021-09-02

申请人： AmberGen, Inc.

发明人： Mark J Lim ,  Vladislav B Bergo ,  Kenneth J Rothschild

IPC分类号： G01N33/543 ,  G01N33/53 ,  G01N33/68

CPC分类号： G01N33/54306 ,  G01N33/5308 ,  G01N33/6845 ,  G01N33/6848

摘要： Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.

公开(公告)号：US11782056B2

公开(公告)日：2023-10-10

申请号：US16839951

申请日：2020-04-03

申请人： AmberGen, Inc.

发明人： Mark J Lim ,  Vladislav B Bergo ,  Kenneth J Rothschild

IPC分类号： G01N33/543 ,  G01N33/68 ,  G01N33/53

CPC分类号： G01N33/54306 ,  G01N33/5308 ,  G01N33/6845 ,  G01N33/6848

摘要： Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.

公开(公告)号：US10060912B2

公开(公告)日：2018-08-28

申请号：US15283694

申请日：2016-10-03

申请人： AmberGen, Inc.

发明人： Mark J Lim ,  Vladislav B Bergo ,  Kenneth J Rothschild

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/68

CPC分类号： G01N33/54306 ,  G01N33/5308 ,  G01N33/6845 ,  G01N33/6848

摘要： Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.

公开(公告)号：US11846634B2

公开(公告)日：2023-12-19

申请号：US16839907

申请日：2020-04-03

申请人： AmberGen, Inc.

发明人： Mark J Lim ,  Vladislav B Bergo ,  Kenneth J Rothschild

IPC分类号： G01N33/543 ,  G01N33/68 ,  G01N33/53

CPC分类号： G01N33/54306 ,  G01N33/5308 ,  G01N33/6845 ,  G01N33/6848

摘要： Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.

公开(公告)号：US10088474B2

公开(公告)日：2018-10-02

申请号：US14876301

申请日：2015-10-06

申请人： AmberGen, Inc.

发明人： Mark J Lim ,  Kenneth J Rothschild

IPC分类号： G01N33/543 ,  C12Q1/6806

摘要： Methods are described for phototransferring a compound from a first surface to a second surface. Compounds are described with photocleavable linkers. Compounds attached to a first surface through a photocleavable linker are put in proximity (or contact) with a second surface, and then phototransferred to the second surface upon exposure to electromagnetic radiation. Illuminating the compound with radiation photocleaves the compound from the first surface and transfers the compound to the second surface.