公开(公告)号：US20120276086A1

公开(公告)日：2012-11-01

申请号：US13449417

申请日：2012-04-18

申请人： Amelia Nancy BLACK ,  David B. PASSMORE ,  Mohan SRINIVASAN ,  Lynn F. DICKEY ,  Kevin M. COX ,  Charles G. PEELE ,  Ming-Bo WANG

发明人： Amelia Nancy BLACK ,  David B. PASSMORE ,  Mohan SRINIVASAN ,  Lynn F. DICKEY ,  Kevin M. COX ,  Charles G. PEELE ,  Ming-Bo WANG

IPC分类号： C07K16/28 ,  C12N5/071 ,  A61P37/04 ,  A61K35/00 ,  A61P35/02 ,  C12N5/10 ,  A61K39/395

CPC分类号： C07K16/2878 ,  A61K2039/505 ,  C07K2317/13 ,  C07K2317/21 ,  C07K2317/41 ,  C07K2317/72 ,  C07K2317/732

摘要： The invention pertains to anti-CD30 antibodies that lack fucosyl and xylosyl residues. The antibodies of the invention exhibit increased antibody-dependent cellular cytotoxicity (ADCC) activity, including the ability to lyse CD30-expressing cell lines that are not lysed by the fucosylated and xylosylated form of the antibodies. The invention also provides host cells that express the anti-CD30 antibodies that lack fucosyl and xylosyl residues, wherein the host cells are deficient for a fucosyltransferase and a xylosyltransferase. Methods of using the antibodies to inhibit the grown of CD30+ cells, such as tumor cells, are also provided.

摘要翻译： 本发明涉及缺乏岩藻糖基和木糖基残基的抗-CD30抗体。 本发明的抗体表现出增加的抗体依赖性细胞毒性（ADCC）活性，包括裂解不被岩藻糖基化和木糖基化形式的抗体裂解的表达CD30的细胞系的能力。 本发明还提供表达缺乏岩藻糖基和木糖基残基的抗CD30抗体的宿主细胞，其中所述宿主细胞对于岩藻糖基转移酶和木糖基转移酶是缺陷的。 还提供了使用抗体抑制生长的CD30 +细胞例如肿瘤细胞的方法。