公开(公告)号：US20140329227A1

公开(公告)日：2014-11-06

申请号：US14353704

申请日：2012-10-25

Applicant: Amgen Inc.

Inventor： Joseph Edward Ssultz ,  Roger Hart ,  Brent Welborn

IPC: G01N1/30

CPC classification number: G01N1/30 ,  A61L2/10 ,  B01D15/24 ,  C07K1/16 ,  C07K1/22

Abstract: Methods of inactivation of a virus in a sample comprising a protein component are provided. Also provided are methods of reducing protein degradation or modification in to the presence of a reactive species, such as a reactive species generated as a result of UV exposure, are also provided. In another aspect, a method of reducing oxidation of methionine residues, tryptophan residues or both methionine and tryptophan residues in a protein subjected to UV light is provided. The disclosed methods can be performed at any scale and can be automated as desired.

Abstract translation: 提供了包含蛋白质组分的样品中病毒失活的方法。 还提供了减少蛋白质降解或修饰反应物质（例如由于UV暴露产生的反应性物质）的存在的方法。 另一方面，提供了在经受紫外光的蛋白质中降低蛋氨酸残基，色氨酸残基或甲硫氨酸和色氨酸残基二氧化的方法。 所公开的方法可以以任何规模执行，并且可以根据需要自动化。

公开(公告)号：US10955291B2

公开(公告)日：2021-03-23

申请号：US14385249

申请日：2013-03-07

Applicant: Amgen Inc.

Inventor： Roger Hart ,  Amanda Lewis ,  Brent Welborn

IPC: G01J1/42 ,  G01N33/52 ,  A61L2/00 ,  A23L3/28 ,  A61L2/10 ,  G01N21/64

Abstract: Methods of determining the dose of UVC light delivered to a sample comprising a low optical transmission complex fluid are provided. Also provided are methods of inactivation of an organism, such as a spore, a bacteria or a virus, in a sample comprising dose of UVC light delivered to a sample comprising a low optical transmission complex fluid.

公开(公告)号：US20150053546A1

公开(公告)日：2015-02-26

申请号：US14385249

申请日：2013-03-07

Applicant: Amgen Inc.

Inventor： Roger Hart ,  Amanda Lewis ,  Brent Welborn

IPC: G01J1/42 ,  G01N33/52 ,  G01N21/64 ,  A61L2/10

CPC classification number: G01J1/429 ,  A23L3/28 ,  A61L2/0035 ,  A61L2/0047 ,  A61L2/10 ,  A61L2202/21 ,  G01N21/6486 ,  G01N33/52 ,  G01N2201/06113

Abstract: Methods of determining the dose of UVC light delivered to a sample comprising a low optical transmission complex fluid are provided. Also provided are methods of inactivation of an organism, such as a spore, a bacteria or a virus, in a sample comprising dose of UVC light delivered to a sample comprising a low optical transmission complex fluid.

Abstract translation: 提供了确定输送到包含低透光复合流体的样品的UVC光的剂量的方法。 还提供了在包含剂量的UVC光的样品中灭活生物体如孢子，细菌或病毒的方法，所述UVC光递送到包含低透光复合流体的样品。