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    Process of high purity albumin production
    2.
    发明授权
    Process of high purity albumin production 有权
    高纯白蛋白生产工艺

    公开(公告)号：US07223561B2

    公开(公告)日：2007-05-29

    申请号：US10873504

    申请日：2004-06-22

    申请人： Andrew Robert Goodey ,  Darell Sleep ,  Hendrik Van Urk ,  Stephen Berezenko ,  John Rodney Woodrow ,  Richard Alan Johnson ,  Patricia Carol Wood ,  Stephen James Burton ,  Alan Victor Quirk

    发明人： Andrew Robert Goodey ,  Darell Sleep ,  Hendrik Van Urk ,  Stephen Berezenko ,  John Rodney Woodrow ,  Richard Alan Johnson ,  Patricia Carol Wood ,  Stephen James Burton ,  Alan Victor Quirk

    IPC分类号： C12N15/14 ,  C07K14/00

    CPC分类号： C07K14/70571 ,  C07K14/765 ,  C07K2319/10

    摘要： A process is provided for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through positive mode cation exchange and then positive mode anion exchange chromatography. Other steps may also be employed, ultrafiltration, gel permeation chromatography, affinity chromatography binding the albumin by using blue dyes) and affinity chromatography binding contaminants by using an aminophenylboronic acid resin. Elution of albumin, with a compound having affinity for albumin, from a material having no specific affinity for albumin is also disclosed, as is removal of ammonium ions with a counter-ion.

    摘要翻译： 提供了一种制备白蛋白的方法，其具有极低水平或基本上不含着色剂，金属离子，人蛋白质，宿主蛋白质，白蛋白片段，白蛋白和病毒的聚合物或聚集体，并且基本上未被糖化 ，游离硫醇相对较高，具有完整的C末端。 该方法包括使白蛋白（优选由转化的酵母表达和分泌）通过阳离子阳离子交换，然后进行阳性阴离子交换层析。 还可以采用其它步骤，超滤，凝胶渗透色谱法，通过使用蓝色染料结合白蛋白的亲和层析）和通过使用氨基苯基硼酸树脂的亲和层析结合污染物。 还公开了从对白蛋白没有特异性亲和力的物质洗脱白蛋白和具有对白蛋白的亲和性的化合物，以及用抗衡离子除去铵离子。