公开(公告)号：US07527814B2

公开(公告)日：2009-05-05

申请号：US11699764

申请日：2007-01-30

申请人： Anil Kumar Singh ,  Suman Preet Singh Khanuja ,  Sudeep Tandon ,  Alok Kalra ,  Deeptanjali Sahoo ,  Atul Prakash Kahol ,  Madan Mohan Gupta ,  Ram Kishor Verma ,  Arun Kumar Kukreja ,  Mansoor Alam ,  Guru Das Bagachi ,  Ravi Prakash Bansal ,  Mahendra Pandurang Darokar ,  Anil Kumar Gupta

发明人： Anil Kumar Singh ,  Suman Preet Singh Khanuja ,  Sudeep Tandon ,  Alok Kalra ,  Deeptanjali Sahoo ,  Atul Prakash Kahol ,  Madan Mohan Gupta ,  Ram Kishor Verma ,  Arun Kumar Kukreja ,  Mansoor Alam ,  Guru Das Bagachi ,  Ravi Prakash Bansal ,  Mahendra Pandurang Darokar ,  Anil Kumar Gupta

IPC分类号： A61K36/00

CPC分类号： A01N65/22 ,  A01N65/00 ,  B01D11/0288 ,  Y02A40/143 ,  A01N2300/00

摘要： The invention provides a simple method for isolation of calliterpenone (16α,17 dihydroxy-3-oxo phyllocladane), a phyllocladane diterpenoid with the plant growth regulating properties, from plant genus Callicarpa.

摘要翻译： 本发明提供了一种用于从植物属Callicarpa分离具有植物生长调节性质的叶绿素二烯酮（16α，17二羟基-3-氧基十二烷）的简单方法。

公开(公告)号：US06589780B2

公开(公告)日：2003-07-08

申请号：US09736434

申请日：2000-12-15

申请人： Suchitra Banerjee ,  Arun Kumar Kukreja ,  Praveen Chandra Verma ,  Atul Prakash Kahol ,  Sushil Kumar

发明人： Suchitra Banerjee ,  Arun Kumar Kukreja ,  Praveen Chandra Verma ,  Atul Prakash Kahol ,  Sushil Kumar

IPC分类号： C12N500

CPC分类号： A01H4/001 ,  C12M27/02 ,  C12M27/20 ,  C12M27/22 ,  C12M29/06

摘要： A bio-reactor for enhancing the biomass yield using a closed sterile vessel having a medium, said reactor comprising a central shaft, a support matrix oriented horizontally and dividing the vessel into an upper and a lower chamber, a means for stirring selected from turbine-2,4,6-impeller; marine blade impeller; helical blade impeller; mounted onto the central shaft and located at a predetermined distance from the support matrix so as to generate both radially and axially directed volumetric flow of the media; and at least two semi-circular spargers located both in the upper and lower compartments of the vessel at a predetermined distance from the support matrix.

摘要翻译： 一种用于使用具有介质的封闭无菌容器提高生物质产量的生物反应器，所述反应器包括中心轴，水平取向并将容器分成上下室的支撑体，用于搅拌的装置， 2,4,6叶轮 船用叶片叶轮; 螺旋叶片叶轮; 安装在中心轴上并且位于与支撑矩阵相距预定距离处，以便产生介质的径向和轴向定向的体积流动; 以及位于容器的上部和下部隔室中的至少两个半圆形喷射器，距离支撑矩阵预定距离。

公开(公告)号：US06365757B1

公开(公告)日：2002-04-02

申请号：US09535767

申请日：2000-03-28

申请人： Sunil Kumar Chattopadhyay ,  Suchitra Banerjee ,  Shipra Agarwal ,  Koneni Venkata Sashidhara ,  Vinayak Tripathi ,  Arun Kumar Kukreja ,  Sushil Kumar ,  Manish Kulshrestha ,  Ram Prakash Sharma ,  Vijay Kumar Mehta

发明人： Sunil Kumar Chattopadhyay ,  Suchitra Banerjee ,  Shipra Agarwal ,  Koneni Venkata Sashidhara ,  Vinayak Tripathi ,  Arun Kumar Kukreja ,  Sushil Kumar ,  Manish Kulshrestha ,  Ram Prakash Sharma ,  Vijay Kumar Mehta

IPC分类号： C07D31104

CPC分类号： C07D311/60

摘要： This invention relates to a process for the production of a compound (+) catechin penta acetate of formula I from Taxus wallichiana tissue cultures which comprises : (a) inoculation of explants on different media compositions supplemented with combinations of auxins (1-5 mg/l) and cytokinins (0.1-1.0 mg/l); (b) incubation of the cultures under continuous light or dark conditions for 4-6 weeks for callus cultures followed by subculturing at 4-6 weeks intervals; (c) harvesting of the calli at different growth phases ranging from 12-36 months; (d) extraction of fresh pulverized calli with polar solvents at room temperature; (e) evaporating the solvent to give a residue; and partitioning of the residue between water and chlorinated solvents and evaporating the solvent to a semisolid mass; (f) subjecting the resultant mass to column chromatography over suitable adsorbent and (g) eluting with organic solvent/mixtures of organic solvents to get (+) catechin penta acetate.

摘要翻译： 本发明涉及一种从紫杉属组织培养物生产式I化合物（+）儿茶素五乙酸酯的方法，该方法包括：（a）将外植体接种在不同的培养基组合物上，所述培养基组合物补充有生长素组合（1-5mg / l）和细胞分裂素（0.1-1.0mg / l）; （b）在连续的光照或黑暗条件下培养培养物4-6周，用于愈伤组织培养，然后以4-6周间隔传代培养; （c）在12-36个月的不同生长阶段收获愈伤组织; （d）在室温下用极性溶剂萃取新鲜的粉状愈伤组织; （e）蒸发溶剂得到残余物; 并将残留物在水和氯化溶剂之间分配并将溶剂蒸发成半固体物质; （f）使所得物质经合适的吸附剂进行柱层析，和（g）用有机溶剂/有机溶剂的混合物洗脱得到（+）儿茶素五乙酸酯。