公开(公告)号：US20060063256A1

公开(公告)日：2006-03-23

申请号：US11268170

申请日：2005-11-07

申请人： Anne Norment ,  Thaddeus George

发明人： Anne Norment ,  Thaddeus George

IPC分类号： C12N5/08

CPC分类号： C12N5/0636 ,  A61K38/18 ,  A61K38/195 ,  G01N33/5091 ,  G01N33/56972

摘要： Regulatory T cell subpopulation (Treg) are isolated for a human host by selection for cells expressing CD4 and CD25. The Treg cells are further characterized by expression of CTLA-4, CCR6, and CD30. In addition, the Treg cells are CD62Lhi, CD45RBlo, CD45ROhi, CD45RA−. The Treg cells of the invention reflect the immunologic status of the donor, in terms of the number, location and T cell antigen receptor specificity of the Treg cells. This information is used in diagnostic assays relating to immunologic disorders, e.g. cancer related immunosuppression; autoimmune disorders; atopic states, etc. The isolated Treg cells are useful in transplantation, for experimental evaluation, and as a source of subset and cell specific products, including mRNA species useful in identifying genes specifically expressed in these cells, and as targets for the discovery of factors or molecules that can affect them. Culture assays and systems of interest include the interactions of Treg cells with immature and mature dendritic cells, interactions with T cell subsets, responsiveness to antigen specific and non-specific stimulus, and the like.

摘要翻译： 通过选择表达CD4和CD25的细胞，为人宿主分离调节性T细胞亚群（Treg）。 Treg细胞的进一步特征在于表达CTLA-4，CCR6和CD30。 另外，Treg细胞是CD62L，CD45RB，CD45RO，CD45RA，CD45RA，CD45RA。 根据Treg细胞的数量，位置和T细胞抗原受体特异性，本发明的Treg细胞反映了供体的免疫状态。 该信息用于与免疫学疾病有关的诊断测定，例如， 癌相关免疫抑制; 自身免疫性疾病 特应性状态等。分离的Treg细胞可用于移植，用于实验评估，并且作为子集和细胞特异性产物的来源，包括用于鉴定在这些细胞中特异性表达的基因的mRNA种类，以及作为发现因子的目标 或可能影响它们的分子。 文化测定和感兴趣的系统包括Treg细胞与未成熟和成熟树突状细胞的相互作用，与T细胞亚群的相互作用，对抗原特异性和非特异性刺激的反应性等。