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    Blood and cell analysis using an imaging flow cytometer
    1.
    发明授权
    Blood and cell analysis using an imaging flow cytometer 有权
    使用成像流式细胞仪进行血液和细胞分析

    公开(公告)号：US08406498B2

    公开(公告)日：2013-03-26

    申请号：US12631795

    申请日：2009-12-04

    申请人： William Ortyn ,  David Basiji ,  Philip Morrissey ,  Thaddeus George ,  Brian Hall ,  Cathleen Zimmerman ,  David Perry

    发明人： William Ortyn ,  David Basiji ,  Philip Morrissey ,  Thaddeus George ,  Brian Hall ,  Cathleen Zimmerman ,  David Perry

    IPC分类号： G06K9/00

    CPC分类号： A61B1/00188 ,  G01N15/147 ,  G01N15/1475 ,  G01N2015/1488 ,  G06K9/0014

    摘要： Multimodal or multispectral images of cells comprising a population of cells are simultaneously collected. Photometric and/or morphometric image features identifiable in the images are used to identify differences between first and second populations of cells. The differences can include changes in a relative percentage of different cell types in each population, or a change in a first type of cell present in the first population of cells and the same type of cell in the second population of cells. The changes may be indicative of a disease state, indicative of a relative effectiveness of a therapy, or indicative of a health of the person from whom the cells populations were obtained.

    摘要翻译： 同时收集包含细胞群的细胞的多峰或多光谱图像。 图像中可识别的光度和/或形态测量图像特征用于识别第一和第二细胞群体之间的差异。 差异可以包括每个群体中不同细胞类型的相对百分比的变化，或第一细胞群体中存在的第一类细胞和第二细胞群体中相同类型的细胞的变化。 这些改变可以指示疾病状态，指示治疗的相对有效性或指示获得细胞群体的人的健康。

    Human regulatory T cells and uses thereof
    2.
    发明申请
    Human regulatory T cells and uses thereof 审中-公开
    人体调节性T细胞及其用途

    公开(公告)号：US20060063256A1

    公开(公告)日：2006-03-23

    申请号：US11268170

    申请日：2005-11-07

    申请人： Anne Norment ,  Thaddeus George

    发明人： Anne Norment ,  Thaddeus George

    IPC分类号： C12N5/08

    CPC分类号： C12N5/0636 ,  A61K38/18 ,  A61K38/195 ,  G01N33/5091 ,  G01N33/56972

    摘要： Regulatory T cell subpopulation (Treg) are isolated for a human host by selection for cells expressing CD4 and CD25. The Treg cells are further characterized by expression of CTLA-4, CCR6, and CD30. In addition, the Treg cells are CD62Lhi, CD45RBlo, CD45ROhi, CD45RA−. The Treg cells of the invention reflect the immunologic status of the donor, in terms of the number, location and T cell antigen receptor specificity of the Treg cells. This information is used in diagnostic assays relating to immunologic disorders, e.g. cancer related immunosuppression; autoimmune disorders; atopic states, etc. The isolated Treg cells are useful in transplantation, for experimental evaluation, and as a source of subset and cell specific products, including mRNA species useful in identifying genes specifically expressed in these cells, and as targets for the discovery of factors or molecules that can affect them. Culture assays and systems of interest include the interactions of Treg cells with immature and mature dendritic cells, interactions with T cell subsets, responsiveness to antigen specific and non-specific stimulus, and the like.

    摘要翻译： 通过选择表达CD4和CD25的细胞，为人宿主分离调节性T细胞亚群（Treg）。 Treg细胞的进一步特征在于表达CTLA-4，CCR6和CD30。 另外，Treg细胞是CD62L，CD45RB，CD45RO，CD45RA，CD45RA，CD45RA。 根据Treg细胞的数量，位置和T细胞抗原受体特异性，本发明的Treg细胞反映了供体的免疫状态。 该信息用于与免疫学疾病有关的诊断测定，例如， 癌相关免疫抑制; 自身免疫性疾病 特应性状态等。分离的Treg细胞可用于移植，用于实验评估，并且作为子集和细胞特异性产物的来源，包括用于鉴定在这些细胞中特异性表达的基因的mRNA种类，以及作为发现因子的目标 或可能影响它们的分子。 文化测定和感兴趣的系统包括Treg细胞与未成熟和成熟树突状细胞的相互作用，与T细胞亚群的相互作用，对抗原特异性和非特异性刺激的反应性等。