公开(公告)号：US5789247A

公开(公告)日：1998-08-04

申请号：US321613

申请日：1994-10-12

申请人： Annick Ballay ,  Georges Boffa ,  Jean-Pierre Cartron ,  Stany Chretien ,  Patrick Lambin ,  Claude Lopez ,  Sylvie Prigent ,  Charles Salmon

发明人： Annick Ballay ,  Georges Boffa ,  Jean-Pierre Cartron ,  Stany Chretien ,  Patrick Lambin ,  Claude Lopez ,  Sylvie Prigent ,  Charles Salmon

IPC分类号： C12N5/22 ,  C12N15/85 ,  C07H21/04 ,  C12N15/63 ,  C12P21/06

CPC分类号： C12N15/85 ,  C12N2840/20

摘要： A plasmid vector designed for transforming lymphoblastoid cells, immortalized by Epstein Barr virus, for producing heterologous proteins, which comprises the bacterial DNA elements required for its amplification together with the following DNA fragments: the DNA elements which constitute an expression cassette allowing the insertion of a heterologous DNA to be expressed in the human lymphoblastoid cells, the Adenovirus 5 derived DNA sequence encoding the VA I and VA II RNA aimed at optimizing the messenger RNA translation, a gene engineered to be efficient as a selective marker in eukaryotic cells, and DNA segments selected for their property to promote plasmid DNA integration into the cell chromosome DNA.

摘要翻译： 设计用于转化由Epstein Barr病毒永生化的淋巴母细胞细胞用于产生异源蛋白的质粒载体，其包含与以下DNA片段一起扩增所需的细菌DNA元件：构成表达盒的DNA元件，其允许插入 在人类淋巴母细胞中表达的异源DNA，编码旨在优化信使RNA翻译的VA I和VA II RNA的腺病毒5衍生的DNA序列，被设计为在真核细胞中作为选择性标记有效的基因和DNA片段 选择其性质来促进质粒DNA整合入细胞染色体DNA。