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1.发明授权Defective recombinant adenovirus lacking E1A coding sequence and comprising a heterologous nucleotide sequence under control of the E1A promoter 失效缺乏E1A编码序列的重组腺病毒缺陷，包含E1A启动子控制下的异源核苷酸序列公开(公告)号：US06426216B1
公开(公告)日：2002-07-30
申请号：US08460802
申请日：1995-06-02
IPC分类号： C12N15861
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10343 , C12N2710/10351 , C12N2730/10122 , C12N2730/10134
摘要： Recombinant DNA modified by a nucleotide sequence coding for a specific polypeptide sequence whose expression is sought, this recombinant DNA being appropriate to the transformation of eucaryotic cell lines, notably human or animal, the endogenous polymerases of which are susceptible of recognizing the adenovirus promoters. The DNA according to the invention is more particularly characterized by the fact that the said insertion nucleotide sequence is placed under the direct control of the early promoter of the E1A region of the genome of adenovirus.
摘要翻译： 通过编码其寻求表达的特异性多肽序列的核苷酸序列修饰的重组DNA，该重组DNA适于转录真核细胞系，特别是人或动物，内源性聚合酶易于识别腺病毒启动子。 根据本发明的DNA更特别的特征在于所述插入核苷酸序列被置于腺病毒基因组的E1A区的早期启动子的直接控制之下。
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2.发明授权Adenoviral vector with a deletion in the E1A coding region expressing a hetorologous protein 失效使用编码HBV S抗原的缺陷性腺病毒在哺乳动物中诱导抗体的方法公开(公告)号：US06696420B1
公开(公告)日：2004-02-24
申请号：US08093901
申请日：1993-07-20
IPC分类号： A61K4800
CPC分类号： C12N15/86 , C07K14/005 , C12N2710/10343 , C12N2710/10351 , C12N2730/10122 , C12N2730/10134
摘要： Recombinant DNA modified by a nucleotide sequence coding for a specific polypeptide sequence whose expression is sought, this recombinant DNA being appropriate to the transformation of eucaryotic cell lines, notably human or animal, the endogenous polymerases of which are susceptible of recognizing the adenovirus promoters. The DNA according to the invention is more particularly characterized by the fact that the said insertion nucleotide sequence is placed under the direct control of the early promoter of the E1A region of the genome of adenovirus.
摘要翻译： 通过编码其寻求表达的特异性多肽序列的核苷酸序列修饰的重组DNA，该重组DNA适于转录真核细胞系，特别是人或动物，内源性聚合酶易于识别腺病毒启动子。 根据本发明的DNA更特别的特征在于所述插入核苷酸序列被置于腺病毒基因组的E1A区的早期启动子的直接控制之下。
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3.发明授权Expression in non-tumoral human lymphoblastoid lines with an integrative vector 失效在具有整合载体的非肿瘤性人类淋巴母细胞系中表达
公开(公告)号：US5789247A
公开(公告)日：1998-08-04
申请号：US321613
申请日：1994-10-12
申请人： Annick Ballay , Georges Boffa , Jean-Pierre Cartron , Stany Chretien , Patrick Lambin , Claude Lopez , Sylvie Prigent , Charles Salmon
发明人： Annick Ballay , Georges Boffa , Jean-Pierre Cartron , Stany Chretien , Patrick Lambin , Claude Lopez , Sylvie Prigent , Charles Salmon
CPC分类号： C12N15/85 , C12N2840/20
摘要： A plasmid vector designed for transforming lymphoblastoid cells, immortalized by Epstein Barr virus, for producing heterologous proteins, which comprises the bacterial DNA elements required for its amplification together with the following DNA fragments: the DNA elements which constitute an expression cassette allowing the insertion of a heterologous DNA to be expressed in the human lymphoblastoid cells, the Adenovirus 5 derived DNA sequence encoding the VA I and VA II RNA aimed at optimizing the messenger RNA translation, a gene engineered to be efficient as a selective marker in eukaryotic cells, and DNA segments selected for their property to promote plasmid DNA integration into the cell chromosome DNA.
摘要翻译： 设计用于转化由Epstein Barr病毒永生化的淋巴母细胞细胞用于产生异源蛋白的质粒载体，其包含与以下DNA片段一起扩增所需的细菌DNA元件：构成表达盒的DNA元件，其允许插入 在人类淋巴母细胞中表达的异源DNA，编码旨在优化信使RNA翻译的VA I和VA II RNA的腺病毒5衍生的DNA序列，被设计为在真核细胞中作为选择性标记有效的基因和DNA片段 选择其性质来促进质粒DNA整合入细胞染色体DNA。
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