公开(公告)号：US20210147846A1

公开(公告)日：2021-05-20

申请号：US17155387

申请日：2021-01-22

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Yu Zhou

IPC: C12N15/113 ,  C12N15/67 ,  H03K19/20

Abstract: Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.

公开(公告)号：US11898144B2

公开(公告)日：2024-02-13

申请号：US17155387

申请日：2021-01-22

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Yu Zhou

IPC: C12N15/113 ,  C12N15/67 ,  H03K19/20

CPC classification number: C12N15/113 ,  C12N15/67 ,  H03K19/20 ,  C12N2310/122 ,  C12N2310/531

Abstract: Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.

公开(公告)号：US20190185856A1

公开(公告)日：2019-06-20

申请号：US16322799

申请日：2017-08-01

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander GREEN ,  Yu Zhou

IPC: C12N15/113 ,  H03K19/20

CPC classification number: C12N15/113 ,  C12N15/67 ,  C12N2310/122 ,  C12N2310/531 ,  H03K19/20

Abstract: Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.

公开(公告)号：US20240200069A1

公开(公告)日：2024-06-20

申请号：US18403466

申请日：2024-01-03

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Yu Zhou

IPC: C12N15/113 ,  C12N15/67 ,  H03K19/20

CPC classification number: C12N15/113 ,  C12N15/67 ,  H03K19/20 ,  C12N2310/122 ,  C12N2310/531

Abstract: Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.

公开(公告)号：US10941401B2

公开(公告)日：2021-03-09

申请号：US16322799

申请日：2017-08-01

Applicant: Arizona Board of Regents on behalf of Arizona State University

Inventor： Alexander Green ,  Yu Zhou

IPC: C12N15/113 ,  C12N15/67 ,  H03K19/20

Abstract: Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA.