公开(公告)号：US09549968B2

公开(公告)日：2017-01-24

申请号：US13514133

申请日：2010-12-07

申请人： Arne Skerra ,  Michaela Gebauer ,  Dominik Hinz ,  Sabine Rauth ,  Gabriele Matschiner ,  Martin Huelsmeyer

发明人： Arne Skerra ,  Michaela Gebauer ,  Dominik Hinz ,  Sabine Rauth ,  Gabriele Matschiner ,  Martin Huelsmeyer

IPC分类号： A61K38/17 ,  C07K14/775 ,  G01N33/566 ,  C07K14/47 ,  A61K38/00

CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K38/1709 ,  A61K47/60 ,  C07K14/775 ,  C07K2319/30 ,  G01N33/566

摘要： The present invention relates to a novel library for the generation of muteins and to novel muteins derived from human lipocalin 2 (Lcn2, hNGAL) and related proteins that bind a given target with detectable affinity. The invention also relates to corresponding nucleic acid molecules encoding such a mutein and to a method for their generation. The invention further relates to a method for producing such a mutein. For example, such muteins may serve to bind and deplete pathological forms of natural biomolecules such as the amyloid beta peptide in Alzheimer's disease or may target the fibronectin extra-domain B, which is associated with tumor neovasculature.

摘要翻译： 本发明涉及用于产生突变蛋白的新文库和衍生自人脂质运载蛋白2（Lcn2，hNGAL）的新型突变蛋白以及以可检测的亲和力结合给定靶的相关蛋白。 本发明还涉及编码这种突变蛋白的相应核酸分子及其产生方法。 本发明还涉及一种生产这种突变蛋白的方法。 例如，这种突变蛋白可用于结合和消耗天然生物分子的病理形式，例如阿尔茨海默病中的淀粉状蛋白β肽，或可靶向与肿瘤新血管生成相关的纤连蛋白结构域外结构域B.

公开(公告)号：US20130079286A1

公开(公告)日：2013-03-28

申请号：US13514133

申请日：2010-12-07

申请人： Arne Skerra ,  Michaela Gebauer ,  Dominik Hinz ,  Sabine Rauth ,  Gabriele Matschiner ,  Martin Huelsmeyer

发明人： Arne Skerra ,  Michaela Gebauer ,  Dominik Hinz ,  Sabine Rauth ,  Gabriele Matschiner ,  Martin Huelsmeyer

IPC分类号： A61K38/17 ,  C07K14/47

CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K38/1709 ,  A61K47/60 ,  C07K14/775 ,  C07K2319/30 ,  G01N33/566

摘要： The present invention relates to a novel library for the generation of muteins and to novel muteins derived from human lipocalin 2 (Lcn2, hNGAL) and related proteins that bind a given target with detectable affinity. The invention also relates to corresponding nucleic acid molecules encoding such a mutein and to a method for their generation. The invention further relates to a method for producing such a mutein. For example, such muteins may serve to bind and deplete pathological forms of natural biomolecules such as the amyloid beta peptide in Alzheimer's disease or may target the fibronectin extra-domain B, which is associated with tumor neovasculature.

摘要翻译： 本发明涉及用于产生突变蛋白的新文库和衍生自人脂质运载蛋白2（Lcn2，hNGAL）的新型突变蛋白以及以可检测的亲和力结合给定靶的相关蛋白。 本发明还涉及编码这种突变蛋白的相应核酸分子及其产生方法。 本发明还涉及一种生产这种突变蛋白的方法。 例如，这种突变蛋白可用于结合和消耗天然生物分子的病理形式，例如阿尔茨海默病中的淀粉状蛋白β肽，或可靶向与肿瘤新血管生成相关的纤连蛋白结构域外结构域B.