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1.发明申请POLYNUCLEOTIDE SEQUENCE OF FRUIT SOFTENING ASSOCIATED A-MANNOSIDASE AND ITS USES FOR ENHANCING FRUIT SHELF LIFE 有权水果相关的A-MANNOSIDASE的多核苷酸序列及其用于提高果实寿命的用途公开(公告)号：US20110239325A1
公开(公告)日：2011-09-29
申请号：US13003203
申请日：2009-07-09
IPC分类号： A01H1/06 , C12N9/24 , C12N15/63 , C12N1/19 , C12N1/21 , C07H21/04 , C07H21/02 , A01N57/16 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8249 , C12N9/2402 , C12Y302/01024
摘要： The present invention provides isolated polynucleotide sequences encoding α-mannosidase. The present invention further provides DNA constructs comprising the polynucleotide sequence coding for α-mannosidase in sense or anti-sense orientation, RNAi contructs, recombinant vectors comprising the constructs, and host cells comprising the recombinant vector. The present invention further provides transgenic plants, plant cells, transgenic progeny and seeds expressing the polynucleotide with reduced α-mannosidase protein accumulation, having enhanced fruit shelf life.
摘要翻译： 本发明提供了编码α-甘露糖苷酶的分离的多核苷酸序列。 本发明还提供了包含编码正义或反义取向中的α-甘露糖苷酶的多核苷酸序列的RNA构建体，RNAi构建体，包含构建体的重组载体和包含重组载体的宿主细胞。 本发明还提供转基因植物，植物细胞，转基因后代和表达具有降低的α-甘露糖苷酶蛋白质积聚的多核苷酸的种子，具有增强的果实保质期。
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2.发明授权Polynucleotide sequence of fruit softening associated A-mannosidase and its uses for enhancing fruit shelf life 有权水果软化相关α-甘露糖苷酶的多核苷酸序列及其用于提高果实保质期的用途公开(公告)号：US08962918B2
公开(公告)日：2015-02-24
申请号：US13003203
申请日：2009-07-09
CPC分类号： C12N15/8249 , C12N9/2402 , C12Y302/01024
摘要： The present invention provides isolated polynucleotide sequences encoding α-mannosidase. The present invention further provides DNA constructs comprising the polynucleotide sequence coding for α-mannosidase in sense or anti-sense orientation, RNAi constructs, recombinant vectors comprising the constructs, and host cells comprising the recombinant vector. The present invention further provides transgenic plants, plant cells, transgenic progeny and seeds expressing the polynucleotide with reduced α-mannosidase protein accumulation, having enhanced fruit shelf life.
摘要翻译： 本发明提供了编码α-甘露糖苷酶的分离的多核苷酸序列。 本发明还提供了包含编码正义或反义取向中的α-甘露糖苷酶的多核苷酸序列的DNA构建体，RNAi构建体，包含构建体的重组载体和包含重组载体的宿主细胞。 本发明还提供转基因植物，植物细胞，转基因后代和表达具有降低的α-甘露糖苷酶蛋白质积聚的多核苷酸的种子，具有增强的果实保质期。
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3.发明申请POLYNUCLEOTIDE SEQUENCE OF FRUIT SOFTENING ASSOCIATED B-D-N- ACETYLHEXOSAMINIDASE AND ITS USES FOR ENHANCING FRUIT SHELF LIFE 有权水果相关的B-D-N-乙酰氨基葡糖苷酶的多核苷酸序列及其用于增加水果寿命的用途公开(公告)号：US20110258739A1
公开(公告)日：2011-10-20
申请号：US13003051
申请日：2009-07-09
CPC分类号： C12N15/8249 , C12N9/2402 , C12Y302/01052
摘要： The present invention provides isolated polynucleotide sequences encoding β-D-N-acetylhexosaminidase. The present invention further provides DNA construct comprising the polynucleotide sequence coding for β-D-N-acetylhexosaminidase in sense or anti-sense orientation, RNAi construct, recombinant vector comprising the construct and host cells comprising the recombinant vector disclosed in the present invention. The present invention further provides transgenic plant, plant cell, transgenic progeny and seeds expressing the polynucleotide with reduced β-D-N-acetylhexosaminidase protein accumulation, having enhanced fruit shelf life
摘要翻译： 本发明提供了编码'-D-N-乙酰基己糖胺酶的分离的多核苷酸序列。 本发明进一步提供了包含编码正义或反义取向中的“-D-N-乙酰基己糖胺酶”的多核苷酸序列的RNA构建体，RNAi构建体，包含构建体的重组载体和包含本发明公开的重组载体的宿主细胞。 本发明进一步提供转基因植物，植物细胞，转基因后代和表达具有降低的-D-N-乙酰基己糖胺酶蛋白质积聚的多核苷酸的种子，具有增强的果实保质期
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4.发明授权Polynucleotide sequence of fruit softening associated B-D- N -acetylhexosaminidase and its uses for enhancing fruit shelf life 有权水果软化相关B-D-乙酰氨基己糖苷酶的多核苷酸序列及其用于提高果实保质期的用途公开(公告)号：US08987556B2
公开(公告)日：2015-03-24
申请号：US13003051
申请日：2009-07-09
CPC分类号： C12N15/8249 , C12N9/2402 , C12Y302/01052
摘要： The present invention provides isolated polynucleotide sequences encoding β-D-N-acetylhexosaminidase. The present invention further provides DNA construct comprising the polynucleotide sequence coding for β-D-N-acetylhexosaminidase in sense or antisense orientation, RNAi construct, recombinant vector comprising the construct and host cells comprising the recombinant vector disclosed in the present invention. The present invention further provides transgenic plant, plant cell, transgenic progeny and seeds expressing the polynucleotide with reduced β-D-N-acetylhexosaminidase protein accumulation, having enhanced fruit shelf life.
摘要翻译： 本发明提供了编码'-D-N-乙酰基己糖胺酶的分离的多核苷酸序列。 本发明进一步提供了包含正义或反义方向的编码'-D-N-乙酰基己糖胺酶的多核苷酸序列的RNA构建体，RNAi构建体，包含构建体的重组载体和包含本发明公开的重组载体的宿主细胞。 本发明进一步提供转基因植物，植物细胞，转基因后代和表达多核苷酸的种子，所述多核苷酸具有降低的-D-N-乙酰基己糖胺酶蛋白质积累，具有增强的果实保质期。
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公开(公告)号：US20120159668A1
公开(公告)日：2012-06-21
申请号：US13393340
申请日：2010-08-30
申请人： Subhra Chakraborty , Niranjan Chakraborty , Asis Datta , Nasheeman Asraf , Swaraj Basu , Papri Nag
发明人： Subhra Chakraborty , Niranjan Chakraborty , Asis Datta , Nasheeman Asraf , Swaraj Basu , Papri Nag
CPC分类号： C12N15/8282 , C07K14/415
摘要： Polynucleotides isolated from chickpea are disclosed herein. The disclosed polynucleotides of the present invention provide genotype-dependent spatial information on the presence and relative abundance of each gene. The transcriptomic analysis of the polynucleotides revealed (649) non-cannonical genes besides many unexpected candidates with known biochemical functions, which have never been associated with pathostress-responsive transcriptome. The polynucleotides disclosed in the present invention can be used as a molecular tool for isolation of novel genes from plants that can be used for plant improvement. Further, the polynucleotide responsible for improving immunity against fungal pathogen in plants is disclosed herein. The present invention also provides a method of improving immunity against fungal pathogen in plants. Transgenic plants exhibiting improved immunity against fungal pathogen are also provided in the present invention.
摘要翻译： 本文公开了从鹰嘴豆分离的多核苷酸。 所公开的本发明的多核苷酸提供关于每个基因的存在和相对丰度的基因型依赖性空间信息。 多核苷酸的转录组学分析显示（649）除了具有已知生物化学功能的许多意想不到的候选物之外的非大炮基因，其从未与病原体反应性转录组相关联。 本发明中公开的多核苷酸可用作用于分离可用于植物改良的植物中的新基因的分子工具。 此外，本文公开了负责提高植物中真菌病原体免疫力的多核苷酸。 本发明还提供了提高植物中真菌病原体免疫力的方法。 在本发明中也提供了表现出对真菌病原体免疫力的改良的转基因植物。
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公开(公告)号：US09163255B2
公开(公告)日：2015-10-20
申请号：US13393340
申请日：2010-08-30
申请人： Subhra Chakraborty , Niranjan Chakraborty , Asis Datta , Nasheeman Asraf , Swaraj Basu , Papri Nag , Manindra Singh
发明人： Subhra Chakraborty , Niranjan Chakraborty , Asis Datta , Nasheeman Asraf , Swaraj Basu , Papri Nag , Manindra Singh
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8282 , C07K14/415
摘要： Polynucleotides isolated from chickpea are disclosed herein. The disclosed polynucleotides of the present invention provide genotype-dependent spatial information on the presence and relative abundance of each gene. The transcriptomic analysis of the polynucleotides revealed (649) non-cannonical genes besides many unexpected candidates with known biochemical functions, which have never been associated with pathostress-responsive transcriptome. The polynucleotides disclosed in the present invention can be used as a molecular tool for isolation of novel genes from plants that can be used for plant improvement. Further, the polynucleotide responsible for improving immunity against fungal pathogen in plants is disclosed herein. The present invention also provides a method of improving immunity against fungal pathogen in plants. Transgenic plants exhibiting improved immunity against fungal pathogen are also provided in the present invention.
摘要翻译： 本文公开了从鹰嘴豆分离的多核苷酸。 所公开的本发明的多核苷酸提供关于每个基因的存在和相对丰度的基因型依赖性空间信息。 多核苷酸的转录组学分析显示（649）除了具有已知生物化学功能的许多意想不到的候选物之外的非大炮基因，其从未与病原体反应性转录组相关联。 本发明中公开的多核苷酸可用作用于分离可用于植物改良的植物中的新基因的分子工具。 此外，本文公开了负责提高植物中真菌病原体免疫力的多核苷酸。 本发明还提供了提高植物中真菌病原体免疫力的方法。 在本发明中也提供了表现出对真菌病原体免疫力的改良的转基因植物。
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7.发明授权Extra-cellular matrix localized ferritin for iron uptake, storage, and stress tolerance 有权细胞外基质局部铁蛋白铁吸收，储存和应力耐受性公开(公告)号：US08163977B2
公开(公告)日：2012-04-24
申请号：US12308141
申请日：2007-06-08
CPC分类号： C12N15/8271 , C07K14/415
摘要： The present invention relates to environmental stress responsive protein ferritin (CaFer1) of chickpea. The invention discloses identification, isolation and cloning of ECM-localized ferritin (CaFer1) of chickpea and its multifunctional role in nutrient uptake, storage and stress tolerance. Comparative proteomic analysis of the chickpea extra-cellular (ECM) was performed to identify novel components of dehydration stress signaling. In addition, the present invention relates a method for producing environmental stress tolerant transgenic plants over-expressing the said CaFer1 gene. The present invention further provides dehydration stress tolerant transgenic plants overexpressing dehydration-responsive extra cellular matrix (ECM) protein ferritin (CaFer1).
摘要翻译： 本发明涉及鹰嘴豆的环境应激反应性蛋白质铁蛋白（CaFer1）。 本发明公开了鹰嘴豆ECM定位铁蛋白（CaFer1）的鉴定，分离和克隆及其在营养摄取，储存和胁迫耐受性中的多功能作用。 进行鹰嘴豆细胞（ECM）的比较蛋白质组学分析，以鉴定脱水应激信号的新成分。 此外，本发明涉及一种用于产生过表达所述CaFer1基因的环境胁迫耐受转基因植物的方法。 本发明还提供了过表达脱水反应性细胞外基质（ECM）蛋白质铁蛋白（CaFer1）的脱水胁迫耐受转基因植物。
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8.发明申请Extra-Cellular Matrix Localized Ferritin for Iron Uptake, Storage, and Stress Tolerance 有权超细胞骨架定位铁蛋白铁吸收，储存和应力容忍公开(公告)号：US20100251426A1
公开(公告)日：2010-09-30
申请号：US12308141
申请日：2007-06-08
IPC分类号： A01H1/00 , C07K14/00 , C07H21/04 , C12N15/74 , C12N1/21 , C12N15/87 , C12N1/19 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8271 , C07K14/415
摘要： The present invention relates to environmental stress responsive protein ferritin (CaFer1) of chickpea. The invention discloses identification, isolation and cloning of ECM-localized ferritin (CaFer1) of chickpea and its multifunctional role in nutrient uptake, storage and stress tolerance. Comparative proteomic analysis of the chickpea extra-cellular (ECM) was performed to identify novel components of dehydration stress signaling. In addition, the present invention relates a method for producing environmental stress tolerant transgenic plants over-expressing the said CaFer1 gene. The present invention further provides dehydration stress tolerant transgenic plants overexpressing dehydration-responsive extra cellular matrix (ECM) protein ferritin (CaFer1).
摘要翻译： 本发明涉及鹰嘴豆的环境应激反应性蛋白质铁蛋白（CaFer1）。 本发明公开了鹰嘴豆ECM定位铁蛋白（CaFer1）的鉴定，分离和克隆及其在营养摄取，储存和胁迫耐受性中的多功能作用。 进行鹰嘴豆细胞（ECM）的比较蛋白质组学分析，以鉴定脱水应激信号的新成分。 此外，本发明涉及一种用于产生过表达所述CaFer1基因的环境胁迫耐受转基因植物的方法。 本发明还提供了过表达脱水反应性细胞外基质（ECM）蛋白质铁蛋白（CaFer1）的脱水胁迫耐受转基因植物。
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