公开(公告)号：US11613761B1

公开(公告)日：2023-03-28

申请号：US15526550

申请日：2015-11-13

Applicant: BASF PLANT SCIENCE COMPANY GMBH ,  BIORIGINAL FOOD & SCIENCE CORPORATION

Inventor： Toralf Senger ,  Laurent Marty ,  Irene Kunze ,  Heiko Haertel ,  Steven Craig Bremmer ,  Steven D. Breazeale ,  Joerg Bauer ,  Patricia Vrinten ,  Sten Stymne ,  Jenny Lindberg Yilmaz ,  John A. McElver ,  Dietrich Rein ,  Carl Andre

IPC: C12N15/82 ,  C12N9/02

Abstract: T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.

公开(公告)号：US10731169B2

公开(公告)日：2020-08-04

申请号：US14902715

申请日：2014-07-03

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Toralf Senger ,  Joerg Bauer

IPC: C12N15/82 ,  C12N15/113

Abstract: The present invention relates to transgenic nucleic acids, expression cassettes, vectors, plant cells, plant organs and plants. The invention also relates to methods for increasing expression or activity of a target gene, particularly in a plant cell or plant organ, and also to uses of recombinant nucleic acids and expression cassettes to increase expression or activity of a target gene or for manufacturing of a vector, plant cell, plant organ or plant. Incidentally, the invention relates to enhancers for achieving increased expression or activity of a target gene, particularly in a plant cell or plant organ, when operably linked to a promoter functional in such plant cell, plant organ or plant. The invention is described herein with reference to the technical field of production of polyunsaturated fatty acids (PUFAs), without being limited to this technical field.For the production of desired molecules in plant cells, e.g. PUFAs, it is frequently required to express a target gene heterologous to the plant cell, or to overexpress a target gene naturally found in said plant cell.

公开(公告)号：US20230293615A1

公开(公告)日：2023-09-21

申请号：US18162300

申请日：2023-01-31

Applicant: BASF PLANT SCIENCE COMPANY GMBH ,  Bioriginal Food & Science Corporation

Inventor： Toralf Senger ,  Laurent Marty ,  Irene Kunze ,  Heiko Haertel ,  Steven Craig Bremmer ,  Steven D. Breazeale ,  Joerg Bauer ,  Patricia Vrinten ,  Sten Stymne ,  Jenny Lindberg Yilmaz ,  John A. McElver ,  Dietrich Rein ,  Carl Andre

IPC: A61K36/31 ,  C12Q1/6895

CPC classification number: A61K36/31 ,  C12Q1/6895 ,  C12N15/8247

Abstract: T-DNA for expression of a target gene in a plant, wherein the T-DNA comprises a left and a right border element and at least one expression cassette comprising a promoter, operatively linked thereto a target gene, and downstream thereof a terminator, wherein the length of the T-DNA, measured from left to right border element and comprising the target gene, has a length of at least 30000 bp.

公开(公告)号：US11033593B2

公开(公告)日：2021-06-15

申请号：US15526443

申请日：2015-11-13

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Toralf Senger ,  Laurent Marty ,  Irene Kunze ,  Dietrich Rein ,  Joerg Bauer ,  Carl Andre

IPC: A61K36/31 ,  C12N15/82 ,  C12Q1/6895 ,  C12N9/02 ,  C12N9/00 ,  A61K31/202

Abstract: The present invention provides transgenic Brassica events LBFLFK and LBFDAU and progeny thereof, and cells, seeds, plants comprising DNA diagnostic for these events. The invention also provides artificial oligonucleotide primers and probes that are diagnostic for the LBFLFK and LBFDAU events and their progeny in a sample, and methods for detecting the presence of the LBFLFK and LBFDAU events and their progeny in a sample. The invention further provides oil and commodity products derived from the LBFLFK and LBFDAU events.

公开(公告)号：US20160168584A1

公开(公告)日：2016-06-16

申请号：US14902715

申请日：2014-07-03

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Toralf Senger ,  Joerg Bauer

IPC: C12N15/82

Abstract: The present invention relates to transgenic nucleic acids, expression cassettes, vectors, plant cells, plant organs and plants. The invention also relates to methods for increasing expression or activity of a target gene, particularly in a plant cell or plant organ, and also to uses of recombinant nucleic acids and expression cassettes to increase expression or activity of a target gene or for manufacturing of a vector, plant cell, plant organ or plant. Incidentally, the invention relates to enhancers for achieving increased expression or activity of a target gene, particularly in a plant cell or plant organ, when operably linked to a promoter functional in such plant cell, plant organ or plant. The invention is described herein with reference to the technical field of production of polyunsaturated fatty acids (PUFAs), without being limited to this technical field.For the production of desired molecules in plant cells, e.g. PUFAs, it is frequently required to express a target gene heterologous to the plant cell, or to overexpress a target gene naturally found in said plant cell.

Abstract translation: 本发明涉及转基因核酸，表达盒，载体，植物细胞，植物器官和植物。 本发明还涉及用于增加靶基因，特别是在植物细胞或植物器官中的表达或活性的方法，以及重组核酸和表达盒的用途以增加靶基因的表达或活性或用于制备 载体，植物细胞，植物器官或植物。 顺便提及，本发明涉及当可操作地连接到在这种植物细胞，植物器官或植物中起作用的启动子时，用于实现靶基因，特别是在植物细胞或植物器官中增加的表达或活性的增强子。 本发明是参照多不饱和脂肪酸（PUFA）的生产技术领域而不受本技术领域的限制。 为了在植物细胞中产生所需的分子，例如。 PUFA通常需要表达与植物细胞异源的靶基因，或过表达在所述植物细胞中天然存在的靶基因。